Liquid chromatography plays a central role in process-scale manufacturing of therapeutic
plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a …

Monoliths have consolidated their position in bioseparation. More than 200 different
applications have been reported in the past two decades and their advantages compared to …

The demand of high-purity plasmid DNA (pDNA) for gene-therapy and genetic vaccination is
still increasing. For the large scale production of pharmaceutical grade plasmids generic …

The diversity of biomolecules present in plasmid DNA (pDNA)-containing extracts and the
structural and chemical similarities between pDNA and impurities are some of the main …

Monolithic columns were introduced in the early 1990s and have become increasingly
popular as efficient stationary phases for most of the important chromatographic separation …

Chromatographic technology is undoubtedly one of the most diverse and powerful
purification methods for downstream process applications. The diversity and quantity of …

Monoliths are an alternative stationary phase format to conventional particle based media
for large biomolecules. Conventional resins suffer from limited capacities and flow rates …

Macroporous-interconnected cryostructures are prepared via cryoprocess approaches,
where polymerization, crosslinking-polymerization, or even chain aggregation is primarily …

The aim of this study is to prepare supermacroporous pseudospecific cryogel which can be
used for the purification of plasmid DNA (pDNA) from bacterial lysate. N-methacryloyl-(l) …

Gene therapy and genetic vaccines promise to revolutionize the treatment of inherited and
acquired diseases. Since viral vectors are generally associated with numerous …