Alkaline proteases are of considerable interest in view of their activity and stability at alkaline
pH. This review describes the proteases that can resist extreme alkaline environments …

Recombinant DNA technology has now made it possible to produce proteins for
pharmaceutical applications. Consequently, proteins produced via biotechnology now …

This review presents a brief overview of the general categories of commercially used
proteases, and critically surveys the successful strategies currently being used to improve …

Microbial alkaline proteases dominate the worldwide enzyme market, accounting for a two-
thirds share of the detergent industry. Although protease production is an inherent property …

Recently, it has become possible to construct proteins to order and this has promoted
interest in learning how to increase their stability. This article describes recent developments …

Force field parameters that use a combination of Lennard‐Jones and electrostatic
interactions are developed for divalent zinc and tested in solution and protein simulations. It …

Publisher Summary The biological function of the zinc is governed by the composition of its
tetrahedral coordination polyhedron in the metalloprotein, and each ligand group that …

The environments of metal ions (Li+, Na+, K+, Ag+, Cs+, Mg2+, Ca2+, Mn2+, Cu2+, Zn2+) in
proteins and other metal-host molecules have been examined. Regardless of the metal and …

Subtilases are members of the family of subtilisin-like serine proteases. Presently,≥ 50
subtilases are known,≥ 40 of which with their complete amino acid sequences. We have …

We have used laboratory evolution methods to enhance the thermostability and activity of
the psychrophilic protease subtilisin S41, with the goal of investigating the mechanisms by …