Microtubule nucleation is regulated by the γ-tubulin ring complex (γTuRC) and related γ-
tubulin complexes, providing spatial and temporal control over the initiation of microtubule …

Tubulin, the subunit protein of microtubules, is an α/β heterodimer. In many organisms, both
α and β exist in numerous isotypic forms encoded by different genes. In addition, both α and …

γ-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y.
Zheng, BM Alberts, and K. Oegema. 1998. J. Cell Biol. 142: 1–12). Here, we report the …

▪ Abstract Nucleation of microtubules by eukaryotic microtubule organizing centers (MTOCs)
is required for a variety of functions, including chromosome segregation during mitosis and …

Although most eukaryotic cells can express multiple isotypes of αβ-tubulin, the significance
of this diversity has not always been apparent. Recent data indicate that particular αβ-tubulin …

The spindle pole body (SPB) is the major microtubule-organizing center of budding yeast
and is the functional equivalent of the centrosome in higher eukaryotic cells. We used fast …

We describe the identification of GIM1/YKE2, GIM2/PAC10, GIM3, GIM4 and GIM5 in a
screen for mutants that are synthetically lethal with tub4‐1, encoding a mutated yeast γ …

We used the dye N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl)
pyridinium dibromide (FM4-64) and a fusion protein, consisting of the green fluorescent …

A highly enriched spindle pole preparation was prepared from budding yeast and
fractionated by SDS gel electrophoresis. Forty-five of the gel bands that appeared enriched …

Microtubule nucleation is the process in which several tubulin molecules interact to form a
microtubule seed. Microtubule nucleation occurs spontaneously in purified tubulin solutions …