Advances in ultrahigh-throughput screening for directed enzyme evolution
U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
A brief history of synthetic biology
The ability to rationally engineer microorganisms has been a long-envisioned goal dating
back more than a half-century. With the genomics revolution and rise of systems biology in …
back more than a half-century. With the genomics revolution and rise of systems biology in …
[HTML][HTML] Generative AI in medical practice: in-depth exploration of privacy and security challenges
Y Chen, P Esmaeilzadeh - Journal of Medical Internet Research, 2024 - jmir.org
As advances in artificial intelligence (AI) continue to transform and revolutionize the field of
medicine, understanding the potential uses of generative AI in health care becomes …
medicine, understanding the potential uses of generative AI in health care becomes …
Adaptive laboratory evolution–principles and applications for biotechnology
M Dragosits, D Mattanovich - Microbial cell factories, 2013 - Springer
Adaptive laboratory evolution is a frequent method in biological studies to gain insights into
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …
Precise and reliable gene expression via standard transcription and translation initiation elements
An inability to reliably predict quantitative behaviors for novel combinations of genetic
elements limits the rational engineering of biological systems. We developed an expression …
elements limits the rational engineering of biological systems. We developed an expression …
Synthetic biology: applications come of age
AS Khalil, JJ Collins - Nature Reviews Genetics, 2010 - nature.com
Synthetic biology is bringing together engineers and biologists to design and build novel
biomolecular components, networks and pathways, and to use these constructs to rewire …
biomolecular components, networks and pathways, and to use these constructs to rewire …
Synthetic circuits integrating logic and memory in living cells
Logic and memory are essential functions of circuits that generate complex, state-dependent
responses. Here we describe a strategy for efficiently assembling synthetic genetic circuits …
responses. Here we describe a strategy for efficiently assembling synthetic genetic circuits …
Engineering an allosteric transcription factor to respond to new ligands
Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …
Synthetic analog computation in living cells
A central goal of synthetic biology is to achieve multi-signal integration and processing in
living cells for diagnostic, therapeutic and biotechnology applications. Digital logic has been …
living cells for diagnostic, therapeutic and biotechnology applications. Digital logic has been …
The ribosome binding site calculator
HM Salis - Methods in enzymology, 2011 - Elsevier
Abstract The Ribosome Binding Site (RBS) Calculator is a design method for predicting and
controlling translation initiation and protein expression in bacteria. The method can predict …
controlling translation initiation and protein expression in bacteria. The method can predict …