Culture-independent methods for studying environmental microorganisms: methods, application, and perspective

C Su, L Lei, Y Duan, KQ Zhang, J Yang - Applied microbiology and …, 2012 - Springer
Since the application of molecular methods, culture-independent methods (CIMs) have been
developed to study microbial communities from various environments. In the past 20 years …

From cultivation to cancer: formation of N-nitrosamines and other carcinogens in smokeless tobacco and their mutagenic implications

SB Stanfill, SS Hecht, AC Joerger… - Critical Reviews in …, 2023 - Taylor & Francis
Tobacco use is a major cause of preventable morbidity and mortality globally. Tobacco
products, including smokeless tobacco (ST), generally contain tobacco-specific N …

Biogas productivity of anaerobic digestion process is governed by a core bacterial microbiota

Y Tao, ME Ersahin, DSM Ghasimi, H Ozgun… - Chemical Engineering …, 2020 - Elsevier
Anaerobic digestion (AD) has been commercially operated worldwide in full scale as a
resource recovery technology underpinning a circular economy. However, problems such as …

Influence of microbiota and metabolites on the quality of tobacco during fermentation

J Li, Y Zhao, Y Qin, H Shi - BMC microbiology, 2020 - Springer
Background To explore the optimum fermentation conditions for tobacco leaves and also
screen the microbiota and metabolites that are beneficial for fermentation. Methods Tobacco …

Production of Highly Active Extracellular Amylase and Cellulase From Bacillus subtilis ZIM3 and a Recombinant Strain With a Potential Application in Tobacco …

J Dai, A Dong, G Xiong, Y Liu, MS Hossain… - Frontiers in …, 2020 - frontiersin.org
In this study, a series of bacteria capable of degrading starch and cellulose were isolated
from the aging flue-cured tobacco leaves. Remarkably, there was a thermophilic bacterium …

Microbial and enzymatic changes in cigar tobacco leaves during air-curing and fermentation

Q Zhang, G Kong, G Zhao, J Liu, H Jin, Z Li… - Applied Microbiology …, 2023 - Springer
Metabolic enzyme activity and microbial composition of the air-curing and fermentation
processes determine the quality of cigar tobacco leaves (CTLs). In this study, we reveal the …

Development of Candida autochthonous starter for cigar fermentation via dissecting the microbiome

Y Jia, Y Liu, W Hu, W Cai, Z Zheng, C Luo… - Frontiers in …, 2023 - frontiersin.org
Introduction The main goal of tobacco fermentation technology is to minimize the alkaloid
content while improving flavor substance content. Methods This study revealed the microbial …

Effects of fermentation chamber temperature on microbes and quality of cigar wrapper tobacco leaves

M Ren, Y Qin, L Zhang, Y Zhao, R Zhang… - Applied Microbiology and …, 2023 - Springer
The natural fermentation of cigar tobacco leaves usually utilizes natural temperature and
humidity for fermentation. Cigars produced in China are often fermented in winter, and the …

Tobacco microbial screening and application in improving the quality of tobacco in different physical states

Y Ning, LY Zhang, J Mai, JE Su, JY Cai, Y Chen… - Bioresources and …, 2023 - Springer
The first-cured tobacco contains macromolecular substances with negative impacts on
tobacco products quality, and must be aged and fermented to mitigate their effects on the …

复合菌种发酵烟叶产酶及挥发性风味物质变化.

帅瑶, 陶菡, 田运霞, 田永锋, 程凯… - Journal of Henan …, 2020 - search.ebscohost.com
为研究菌株混合发酵对烟叶品质的影响, 通过使用解淀粉芽孢杆菌GUHP86 与GZU03
复配菌种对大理红大CL314 烟叶进行人工控制发酵, 对发酵工艺进行探究, 采用气相 …