Molecular imaging is revolutionizing the way we study the inner workings of the human
body, diagnose diseases, approach drug design, and assess therapies. The field as a whole …

Bioluminescence (BL) is a spectacular phenomenon involving light emission by live
organisms. It is caused by the oxidation of a small organic molecule, luciferin, with molecular …

FRET–Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed
primer on all aspects of the topic, this ready reference reflects the incredible expansion in …

Fluorescence has long been recognized as a powerful tool for probing biological structure
and function. Nearly sixty-five years ago, Coons and colleagues introduced the first …

Successful evolutionary enzyme engineering requires a high throughput screening or
selection method, which considerably increases the chance of obtaining desired properties …

Bioluminescence imaging (BLI) exploits the light-emitting properties of luciferase enzymes
for monitoring cells and biomolecular processes in living subjects. Luciferases can be …

Background Positron emission tomography (PET) allows sensitive, non-invasive analysis of
the distribution of radiopharmaceutical tracers labeled with positron (β+)-emitting …

Fluorescent proteins are widely used to study molecular and cellular events, yet this
traditionally relies on delivery of excitation light, which can trigger autofluorescence …

Cell death is a critically important biological process. Disruption of homeostasis, either by
excessive or deficient cell death, is a hallmark of many pathological conditions. Recent …

Bioluminescence is a ubiquitous imaging modality for visualizing biological processes in
vivo. This technique employs visible light and interfaces readily with most cell and tissue …