For many years, it was generally accepted that mRNA is too unstable to be efficiently used
for gene therapy purposes. In the last decade, however, several research groups faced this …

Nonviral gene delivery is limited to a large extent by multiple extracellular and intracellular
barriers. One of the major barriers, especially in nondividing cells, is the nuclear envelope …

Site-directed RNA editing (SDRE) is a general strategy for making targeted base changes in
RNA molecules. Although the approach is relatively new, several groups, including our own …

Low levels of gene transfer and a lack of tissue-specific targeting of vectors have limited the
therapeutic potential of non-viral gene therapy. This is due to the numerous cellular barriers …

It is well established that cationic peptides can enter cells following attachment to
polyanionic membrane components. We report that the basic nuclear localization signal …

Site-directed RNA editing (SDRE) technologies have great potential for treating genetic
diseases caused by point mutations. Our group and other researchers have developed …

The white-shrimp Litopenaeus schmitti distributes in West Atlantic Ocean, occurring along all
Brazilian coast. Population structure in the Baixada Santista region was identified from …

One of the major obstacles in non-viral gene transfer is the nuclear membrane. Attempts to
improve the transport of DNA to the nucleus through the use of nuclear localization signals …

Cultivated crustacean meat (CCM) is a means to create highly valued shrimp, lobster, and
crab products directly from stem cells, thus removing the need to farm or fish live animals …

Transfection is a powerful tool useful for studying gene function. Establishing transfection
methods that enable highly efficient DNA uptake has become increasingly important. The …