The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

The investigation of biological processes by chemical methods, commonly referred to as
chemical biology, often requires chemical access to biologically relevant macromolecules …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

This review describes recent changes and progress in the field of polymer bioconjugation (ie
covalent attachment of synthetic polymers to biological entities such as nucleic acids …

▪ Abstract Proteins have become accessible targets for chemical synthesis. The basic
strategy is to use native chemical ligation, Staudinger ligation, or other orthogonal chemical …

Among the techniques employed for coupling peptide segments,[1] native chemical ligation
is among the most useful.[2] Since its discovery it has become a powerful tool for the …

Selective incorporation of unnatural amino acids into proteins is a powerful tool for
illuminating the principles of protein design. In particular, fluorinated amino acids have …

A chemo-and regioselective auxiliary-mediated peptide ligation has been developed that is
effective under nonidealized conditions for the synthesis of proteins. This general amide …

Abstract (Chemical Equation Presented) Lighting up ubiquitylation: A photolytically
removable ligation auxiliary (Aux) was employed for the site-specific attachment of ubiquitin …

Native chemical ligation enables the chemical synthesis of proteins. Previously, thiol‐
containing auxiliary groups have been used to extend the reaction scope beyond N‐terminal …