Microbiome engineering: synthetic biology of plant-associated microbiomes in sustainable agriculture
J Ke, B Wang, Y Yoshikuni - Trends in Biotechnology, 2021 - cell.com
To support an ever-increasing population, modern agriculture faces numerous challenges
that pose major threats to global food and energy security. Plant-associated microbes, with …
that pose major threats to global food and energy security. Plant-associated microbes, with …
Industrial biotechnology of Pseudomonas putida: advances and prospects
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …
Inhibition of CRISPR-Cas9 with bacteriophage proteins
Bacterial CRISPR-Cas systems utilize sequence-specific RNA-guided nucleases to defend
against bacteriophage infection. As a countermeasure, numerous phages are known that …
against bacteriophage infection. As a countermeasure, numerous phages are known that …
A compact Cascade–Cas3 system for targeted genome engineering
CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …
A periplasmic phospholipase that maintains outer membrane lipid asymmetry in Pseudomonas aeruginosa
RL Guest, MJ Lee, W Wang… - Proceedings of the …, 2023 - National Acad Sciences
The outer membrane of Gram-negative bacteria is unique in both structure and function. The
surface-exposed outer leaflet is composed of lipopolysaccharide, while the inner leaflet is …
surface-exposed outer leaflet is composed of lipopolysaccharide, while the inner leaflet is …
Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange
Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single …
describes the use of this technique to make gene knockouts and knock-ins, as well as single …
The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes
Abstract The 'Standard European Vector Architecture'database (SEVA-DB, http://seva. cnb.
csic. es) was conceived as a user-friendly, web-based resource and a material clone …
csic. es) was conceived as a user-friendly, web-based resource and a material clone …
Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi
The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …
genetic tools needed to systematically associate genes with phenotypes. This is the major …
Biofilm development and enhanced stress resistance of a model, mixed-species community biofilm
Most studies of biofilm biology have taken a reductionist approach, where single-species
biofilms have been extensively investigated. However, biofilms in nature mostly comprise …
biofilms have been extensively investigated. However, biofilms in nature mostly comprise …
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and …
KH Choi, A Kumar, HP Schweizer - Journal of microbiological methods, 2006 - Elsevier
A rapid microcentrifuge-based method is described for preparation of Pseudomonas
aeruginosa electrocompetent cells with up to 10,000-fold increased transformation …
aeruginosa electrocompetent cells with up to 10,000-fold increased transformation …