Bacterial origins of human cell-autonomous innate immune mechanisms

T Wein, R Sorek - Nature Reviews Immunology, 2022 - nature.com
The cell-autonomous innate immune system enables animal cells to resist viral infection.
This system comprises an array of sensors that, after detecting viral molecules, activate the …

Bacteria deplete deoxynucleotides to defend against bacteriophage infection

N Tal, A Millman, A Stokar-Avihail, T Fedorenko… - Nature …, 2022 - nature.com
DNA viruses and retroviruses consume large quantities of deoxynucleotides (dNTPs) when
replicating. The human antiviral factor SAMHD1 takes advantage of this vulnerability in the …

Phage defense origin of animal immunity

BR Morehouse - Current Opinion in Microbiology, 2023 - Elsevier
Highlights•Phage and bacterial warfare resulted in diverse immune defense
strategies.•Animals and bacteria surprisingly have many innate immune processes in …

First Characterization of a Hafnia Phage Reveals Extraordinarily Large Burst Size and Unusual Plaque Polymorphism

L Pan, D Li, Z Sun, W Lin, B Hong, W Qin, L Xu… - Frontiers in …, 2022 - frontiersin.org
A unique lytic phage infecting Hafnia paralvei was isolated and identified. Hafnia phage Ca
belongs to the family Autographiviridae, possessing an icosahedral head with a diameter of …

Single-stranded nucleic acids bind to the tetramer interface of SAMHD1 and prevent formation of the catalytic homotetramer

KJ Seamon, NN Bumpus, JT Stivers - Biochemistry, 2016 - ACS Publications
Sterile alpha motif and HD domain protein 1 (SAMHD1) is a unique enzyme that plays
important roles in nucleic acid metabolism, viral restriction, and the pathogenesis of …

Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase

BP Klemm, D Singh, CE Smith… - Proceedings of the …, 2022 - National Acad Sciences
Levels of the cellular dNTPs, the direct precursors for DNA synthesis, are important for DNA
replication fidelity, cell cycle control, and resistance against viruses. Escherichia coli …

High-resolution structures of the SAMHD1 dGTPase homolog from Leeuwenhoekiella blandensis reveal a novel mechanism of allosteric activation by dATP

BP Klemm, AP Sikkema, AL Hsu, JC Horng… - Journal of Biological …, 2022 - ASBMB
Deoxynucleoside triphosphate (dNTP) triphosphohydrolases (dNTPases) are important
enzymes that may perform multiple functions in the cell, including regulating the dNTP pools …

Antiviral defense via nucleotide depletion in bacteria

N Tal, A Millman, A Stokar-Avihail, T Fedorenko… - Biorxiv, 2021 - biorxiv.org
DNA viruses and retroviruses need to consume large quantities of deoxynucleotides
(dNTPs) when replicating within infected cells. The human antiviral factor SAMHD1 takes …

The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity

CO Barnes, Y Wu, J Song, G Lin… - Proceedings of the …, 2019 - National Acad Sciences
Deoxynucleotide triphosphohydrolases (dNTPases) play a critical role in cellular survival
and DNA replication through the proper maintenance of cellular dNTP pools. While the vast …

Structure of Ddi2, a highly inducible detoxifying metalloenzyme from Saccharomyces cerevisiae

J Li, Y Jia, A Lin, M Hanna, L Chelico, W Xiao… - Journal of Biological …, 2019 - ASBMB
Cyanamide (H 2 N-CN) is used to break bud dormancy in woody plants and to deter alcohol
use in humans. The biological effects of cyanamide in both these cases require the enzyme …