The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

CRISPR/Cas9 technology has become the most examined gene editing technology in
recent years due to its simple design, yet low cost, high efficiency, and simple operation …

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …

Transgene-free plant genome editing in the T0 generation is highly desirable but
challenging,. Here we achieved such a goal using a co-editing strategy via Agrobacterium …

Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

Plants have evolved complex defence mechanisms to avoid invasion of potential pathogens.
Despite this, adapted pathogens deploy effector proteins to manipulate host susceptibility …

Key message We have developed multiplex genome editing toolkits for citrus that
significantly improve citrus genome editing efficacy. Abstract CRISPR/Cas systems have …

Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …

Citrus canker caused by Xanthomonas citri subsp. citri is one of the most devastating citrus
diseases worldwide. Generating disease-resistant citrus varieties is considered one of the …

Fruit tree crops are an essential part of the food production systems and are key to achieve
food and nutrition security. Genetic improvement of fruit trees by conventional breeding has …