Gene therapy has long held promise to correct a variety of human diseases and defects.
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …

To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Therapeutic applications of nuclease-based genome editing would benefit from improved
methods for transgene integration via homology-directed repair (HDR). To improve HDR …

Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a
desirable alternative to insertions at random sites achieved through conventional methods …

The discovery of induced pluripotent stem cells (iPSCs) has made an invaluable contribution
to the field of regenerative medicine, paving way for identifying the true potential of human …

Immunotherapy with T cell modified with gamma-retroviral or lentiviral (LV) vectors to
express a chimeric antigen receptor (CAR) has shown remarkable efficacy in clinical trials …

Cancer therapy has entered a new era, transitioning from unspecific chemotherapeutic
agents to increasingly specific immune-based therapeutic strategies. Among these, chimeric …

Current approaches for inserting autonomous transgenes into the genome, such as CRISPR–
Cas9 or virus-based strategies, have limitations including low efficiency and high risk of …

Zinc-finger nuclease (ZFN)-based in vivo genome editing is a novel treatment that can
potentially provide lifelong protein replacement with single intravenous administration …

Central to the core principle of cell theory, depicting cells' history, state and fate is a
fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq …