A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

JPJ Chen, KE Schmidt, JCH Spence, RA Kirian - Ultramicroscopy, 2021 - Elsevier
We develop an algorithm capable of imaging a three-dimensional object given a collection
of two-dimensional images of that object that are significantly influenced by the curvature of …

[HTML][HTML] Ghostbuster: A phase retrieval diffraction tomography algorithm for cryo-EM

J Yeo, BJ Daurer, D Kimanius, D Balakrishnan… - Ultramicroscopy, 2024 - Elsevier
Ewald sphere curvature correction, which extends beyond the projection approximation,
stretches the shallow depth of field in cryo-EM reconstructions of thick particles. Here we …

[PDF][PDF] Dynamical scattering in ice-embedded proteins in conventional and scanning transmission electron microscopy

ML Leidl, C Sachse, K Müller-Caspary - IUCrJ, 2023 - journals.iucr.org
Structure determination of biological macromolecules using cryogenic electron microscopy
is based on applying the phase object (PO) assumption and the weak phase object (WPO) …

Scattering matrix determination in crystalline materials from 4D scanning transmission electron microscopy at a single defocus value

SD Findlay, HG Brown, PM Pelz… - Microscopy and …, 2021 - academic.oup.com
Recent work has revived interest in the scattering matrix formulation of electron scattering in
transmission electron microscopy as a stepping stone toward atomic-resolution structure …

Unified fast reconstruction algorithm for conventional, phase-contrast, and diffraction tomography

TE Gureyev, HG Brown, HM Quiney, LJ Allen - JOSA A, 2022 - opg.optica.org
A unified method for three-dimensional reconstruction of objects from transmission images
collected at multiple illumination directions is described. The method may be applicable to …

Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells

PN Petrov, H Müller, RM Glaeser - Journal of structural biology, 2022 - Elsevier
In principle, electron cryo-tomography (cryo-ET) of thin portions of cells provides high-
resolution images of the three-dimensional, spatial arrangement of all members of the …

Relative roles of multiple scattering and Fresnel diffraction in the imaging of small molecules using electrons, Part II: Differential Holographic Tomography

TE Gureyev, HM Quiney, A Kozlov, DM Paganin… - Ultramicroscopy, 2021 - Elsevier
It has been argued that in atomic-resolution transmission electron microscopy (TEM) of
sparse weakly scattering structures, such as small biological molecules, multiple electron …

A method for high-resolution three-dimensional reconstruction with Ewald sphere curvature correction from transmission electron images

TE Gureyev, DM Paganin, HG Brown… - Microscopy and …, 2022 - cambridge.org
A method for three-dimensional reconstruction of objects from defocused images collected
at multiple illumination directions in high-resolution transmission electron microscopy is …