Transcriptional Regulation in Saccharomyces cerevisiae: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and …
S Hahn, ET Young - Genetics, 2011 - academic.oup.com
Here we review recent advances in understanding the regulation of mRNA synthesis in
Saccharomyces cerevisiae. Many fundamental gene regulatory mechanisms have been …
Saccharomyces cerevisiae. Many fundamental gene regulatory mechanisms have been …
Current strategies for protein production and purification enabling membrane protein structural biology
Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …
Mechanism of coupled folding and binding of an intrinsically disordered protein
Protein folding and binding are analogous processes, in which the protein 'searches' for
favourable intramolecular or intermolecular interactions on a funnelled energy landscape …
favourable intramolecular or intermolecular interactions on a funnelled energy landscape …
Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation
Cytokinetic abscission, the last step of cell division, is regulated by the ESCRT machinery. In
response to mitotic errors, ESCRT proteins, namely, ALIX, CHMP4B, and CHMP4C …
response to mitotic errors, ESCRT proteins, namely, ALIX, CHMP4B, and CHMP4C …
[HTML][HTML] Allosteric effects of the oncogenic RasQ61L mutant on Raf-RBD
Summary The Ras/Raf/MEK/ERK signal transduction pathway is a major regulator of cell
proliferation activated by Ras-guanosine triphosphate (GTP). The oncogenic mutant …
proliferation activated by Ras-guanosine triphosphate (GTP). The oncogenic mutant …
Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli
M Hammarström, N Hellgren, S van Den Berg… - Protein …, 2002 - Wiley Online Library
A prerequisite for structural genomics and related projects is to standardize the process of
gene overexpression and protein solubility screening to enable automation for higher …
gene overexpression and protein solubility screening to enable automation for higher …
Polyionic tags as enhancers of protein solubility in recombinant protein expression
V Paraskevopoulou, FH Falcone - Microorganisms, 2018 - mdpi.com
Since the introduction of recombinant protein expression in the second half of the 1970s, the
growth of the biopharmaceutical field has been rapid and protein therapeutics has come to …
growth of the biopharmaceutical field has been rapid and protein therapeutics has come to …
The acidic transcription activator Gcn4 binds the mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex
PS Brzovic, CC Heikaus, L Kisselev, R Vernon… - Molecular cell, 2011 - cell.com
The structural basis for binding of the acidic transcription activator Gcn4 and one activator-
binding domain of the Mediator subunit Gal11/Med15 was examined by NMR. Gal11 …
binding domain of the Mediator subunit Gal11/Med15 was examined by NMR. Gal11 …
Exploring weak, transient protein–protein interactions in crowded in vivo environments by in-cell nuclear magnetic resonance spectroscopy
Q Wang, A Zhuravleva, LM Gierasch - Biochemistry, 2011 - ACS Publications
Biology relies on functional interplay of proteins in the crowded and heterogeneous
environment inside cells, and functional protein interactions are often weak and transient …
environment inside cells, and functional protein interactions are often weak and transient …
Structural duality enables a single protein to act as a toxin–antidote pair for meiotic drive
In sexual reproduction, selfish genetic elements known as killer meiotic drivers (KMDs) bias
inheritance by eliminating gametes that do not carry them. The selective killing behavior of …
inheritance by eliminating gametes that do not carry them. The selective killing behavior of …