How does ultrasound‐assisted ionic liquid treatment affect protein? A comprehensive review of their potential mechanisms, safety evaluation, and physicochemical …

W Zhang, ID Boateng, J Xu - … in Food Science and Food Safety, 2024 - Wiley Online Library
Proteins are essential to human health with enormous food applications. Despite their
advantages, plant and animal proteins often exhibit limited molecular flexibility and poor …

Insights into protein-ionic liquid interaction: A comprehensive overview on theoretical and experimental approaches

A Tarannum, JR Rao, NN Fathima - International Journal of Biological …, 2022 - Elsevier
Owing to highly tunable nature, ionic liquids are nesting stance in the scientific community
for a wide variety of applications ranging from electrochemistry to product purification, from …

Efficiency of polymer/salt aqueous two-phase electrophoresis system for recovery of extracellular Kytococcus sedentarius TWHKC01 keratinase

PE Kee, JCW Lan, HS Yim, YH Chow, PT Chen… - Process …, 2021 - Elsevier
Keratinase is widely applied in the bioconversion of feather waste to produce value-added
bioproducts. The wide applications of industrial keratinase have urged the advancement in …

Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its …

ZJ Ng, HS Ng, PE Kee, CY Gan, AMA Mokhtar… - Journal of the Taiwan …, 2024 - Elsevier
Background Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory
effect against oral pathogens. To apply in oral care products, high purity of BLIS is required …

Expression of His-tagged NADPH-dependent acetoacetyl-CoA reductase in recombinant Escherichia coli BL-21 (DE3)

PE Kee, YC Chiang, HS Ng, JCW Lan - Journal of bioscience and …, 2023 - Elsevier
Highlights•The His-tagged phaB was successfully expressed in recombinant Escherichia
coli BL-21 (DE3).•Affinity chromatography was applied for the purification of His-tagged …

Incorporation of electric fields to ionic liquids-based aqueous biphasic system for enhanced recovery of extracellular Kytococcus sedentarius TWHKC01 keratinase

PE Kee, HS Yim, A Kondo, SYW Wong, PT Chen… - Journal of the Taiwan …, 2021 - Elsevier
Background The purification of keratinase is essential to enhance the catalytic efficiency of
keratinase enzyme for industrial applications. Biphasic system incorporated with electric …

Recovery efficiency of a hydrophilic ionic-liquid aqueous biphasic system for the primary purification of cytochrome c from simulated Saccharomyces cerevisiae …

YY Lin, PE Kee, HS Ng, JCW Lan - Process biochemistry, 2020 - Elsevier
Ionic liquid-based aqueous biphasic system (ILABS) has emerged as an attractive green
approach for the extraction and separation of various biomolecules. The growing market …

Evaluation of ionic liquids/salt aqueous biphasic flotation system on recovery of Kytococcus sedentarius TWHKC01 keratinase from crude feedstock

PE Kee, HS Yim, A Kondo, SYW Wong… - Journal of the Taiwan …, 2022 - Elsevier
Background Ionic liquids (IL) is a unique environmental benign solvent with attractive
characteristics which can be used in the formation of aqueous biphasic system. Aqueous …

Considerations in designing a multiphase separation system

S Raguraman, WY Chia, KW Chew, PL Show - Principles of Multiple-Liquid …, 2023 - Elsevier
The recovery of biomolecules such as proteins and nucleic acids can be fairly complicated
as the molecules are fragile and easily susceptible to denaturation. Multiphase liquid …

Evaluation of Aqueous Biphasic Electrophoresis System Based on Halide-Free Ionic Liquids for Direct Recovery of Keratinase

PE Kee, HS Yim, A Kondo, JCW Lan, HS Ng - Marine Drugs, 2021 - mdpi.com
Aqueous biphasic electrophoresis system (ABES) incorporates electric fields into the
biphasic system to separate the target biomolecules from crude feedstock. Ionic liquid (IL) is …