Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description

B Lebas, I Adams, M Al Rwahnih, S Baeyen… - EPPO …, 2022 - Wiley Online Library
High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous
detection and potential identification of any organisms present in a sample. The growing …

Discrepancies between prokaryotes and eukaryotes need to be considered in soil DNA‐based studies

E Lara, D Singer, S Geisen - Environmental Microbiology, 2022 - Wiley Online Library
Metabarcoding approaches are exponentially increasing our understanding of soil
biodiversity, with a major focus on the bacterial part of the microbiome. Part of the soil …

Contrasting seasonal patterns in diet and dung‐associated invertebrates of feral cattle and horses in a rewilding area

EE Thomassen, EE Sigsgaard, MR Jensen… - Molecular …, 2023 - Wiley Online Library
Trophic rewilding is increasingly applied in restoration efforts, with the aim of reintroducing
the ecological functions provided by large‐bodied mammals and thereby promote self …

Nematode mitochondrial metagenomics: A new tool for biodiversity analysis

EM Gendron, JL Sevigny, I Byiringiro… - Molecular Ecology …, 2023 - Wiley Online Library
DNA barcoding approaches have greatly increased our understanding of biodiversity on the
planet, and metabarcoding is widely used for classifying members of the phylum Nematoda …

[HTML][HTML] Impacts of long-term organic production on soil fauna in boreal dairy and cereal farming

M Hagner, I Pohjanlehto, V Nuutinen, H Setälä… - Applied Soil …, 2023 - Elsevier
Intensified arable farming results in fewer functional groups of soil biota with decreased
biodiversity. Organic farming with slurry fertilization and long crop rotation cycles favors soil …

[HTML][HTML] Extracting DNA from soil or directly from isolated nematodes indicate dissimilar community structure for Europe-wide forest soils

J Donhauser, MJI Briones, J Mikola, DL Jones… - Soil Biology and …, 2023 - Elsevier
Nematodes are numerous in soils and play a crucial role in soil food-webs. DNA
metabarcoding offers a time-effective alternative to morphology-based assessments of …

In silico assessment of 18S rDNA metabarcoding markers for the characterization of nematode communities

GF Ficetola, A Guerrieri, I Cantera, A Bonin - Plos one, 2024 - journals.plos.org
Nematodes are keystone actors of soil, freshwater and marine ecosystems, but the
complexity of morphological identification has limited broad-scale monitoring of nematode …

High trophic level organisms and the complexity of soil micro-food webs at aggregate scale regulate carbon accumulation in cropland soils

Z Yao, C Huang, H Hu, T Wang, Y Li, X Sun… - Agriculture, Ecosystems …, 2024 - Elsevier
Enhancing soil organic carbon (SOC) levels via improved fertilization strategies can promote
soil health and support sustainable crop production. While bacteria and fungi play a key role …

Evaluation of metabarcoding primers for analysis of soil nematode communities

MM Sikder, M Vestergård, R Sapkota, T Kyndt… - Diversity, 2020 - mdpi.com
While recent advances in next-generation sequencing technologies have accelerated
research in microbial ecology, the application of high throughput approaches to study the …

Development and application of a DNA metabarcoding method for comprehensive analysis of soil nematode communities

M Kawanobe, K Toyota, K Ritz - Applied Soil Ecology, 2021 - Elsevier
Nematodes mediate soil ecosystem functions together with other soil biota and are
employed as effective ecological indicators. Among nematodes, plant-parasitic nematodes …