Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review

Z Yang, Z Zhang - Biotechnology advances, 2018 - Elsevier
Pichia pastoris has been recognized as one of the most industrially important hosts for
heterologous protein production. Despite its high protein productivity, the optimization of P …

Recombinant protein production in Pichia pastoris: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling

BG Ergün, J Berrios, B Binay, P Fickers - FEMS Yeast Research, 2021 - academic.oup.com
Pichia pastoris is one of the most widely used host for the production of recombinant
proteins. Expression systems that rely mostly on promoters from genes encoding alcohol …

Rhizopus oryzae Lipase, a Promising Industrial Enzyme: Biochemical Characteristics, Production and Biocatalytic Applications

J López-Fernández, MD Benaiges, F Valero - Catalysts, 2020 - mdpi.com
Lipases are biocatalysts with a significant potential to enable a shift from current pollutant
manufacturing processes to environmentally sustainable approaches. The main reason of …

Lipase‐catalyzed process for biodiesel production: Protein engineering and lipase production

HT Hwang, F Qi, C Yuan, X Zhao… - Biotechnology and …, 2014 - Wiley Online Library
Biodiesel is an environment‐friendly and renewable fuel produced by transesterification of
various feedstocks. Although the lipase‐catalyzed biodiesel production has many …

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

MA Nieto-Taype, X Garcia-Ortega, J Albiol… - … in bioengineering and …, 2020 - frontiersin.org
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is currently considered one
of the most promising hosts for recombinant protein production (RPP) and metabolites due …

Biodiesel production from crude Jatropha oil catalyzed by non-commercial immobilized heterologous Rhizopus oryzae and Carica papaya lipases

J Rodrigues, A Canet, I Rivera, NM Osório… - Bioresource …, 2016 - Elsevier
The aim of this study was to evaluate the feasibility of biodiesel production by
transesterification of Jatropha oil with methanol, catalyzed by non-commercial sn-1, 3 …

[HTML][HTML] Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase

SH Duarte, GL del Peso Hernández, A Canet… - Bioresource …, 2015 - Elsevier
The recombinant Rhizopus oryzae lipase (1–3 positional selective), immobilized on
Relizyme OD403, has been applied to the production of biodiesel using single cell oil from …

Lipases from the genus Rhizopus: Characteristics, expression, protein engineering and application

XW Yu, Y Xu, R Xiao - Progress in Lipid Research, 2016 - Elsevier
Lipases are versatile catalysts that hydrolyze ester bonds of water-insoluble glycerides or
carry out reversible reactions at the water/lipid interface. The remarkable characteristics of …

[HTML][HTML] Second-and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase: Influence of the support, substrate acidity and …

J López-Fernández, MD Benaiges, F Valero - Bioresource Technology, 2021 - Elsevier
Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate
(Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for …

Fed-batch operational strategies for recombinant Fab production with Pichia pastoris using the constitutive GAP promoter

X Garcia-Ortega, P Ferrer, JL Montesinos… - Biochemical engineering …, 2013 - Elsevier
Carbon source and growth rate are two major parameters affecting recombinant protein
production in Pichia pastoris. The effect of the most commonly used carbon sources …