Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions

A Henrickson, GE Gorbet, A Savelyev, M Kim… - Analytical …, 2022 - Elsevier
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made
possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic …

A comprehensive review of methods to study lncRNA–protein interactions in solution

MD Badmalia, H Sette Pereira… - Biochemical Society …, 2022 - portlandpress.com
The long non-coding RNAs (lncRNAs) other than rRNA and tRNA were earlier assumed to
be 'junk genomic material'. However, recent advancements in genomics methods have …

Density matching multi-wavelength analytical ultracentrifugation to measure drug loading of lipid nanoparticle formulations

A Henrickson, JA Kulkarni, J Zaifman, GE Gorbet… - ACS …, 2021 - ACS Publications
Previous work suggested that lipid nanoparticle (LNP) formulations, encapsulating nucleic
acids, display electron-dense morphology when examined by cryogenic-transmission …

[HTML][HTML] Determination of the two-dimensional distributions of gold nanorods by multiwavelength analytical ultracentrifugation

SE Wawra, L Pflug, T Thajudeen, C Kryschi… - Nature …, 2018 - nature.com
Abstract Properties of nanoparticles are influenced by various parameters like size, shape or
composition. Comprehensive high throughput characterization techniques are urgently …

Advanced multiwavelength detection in analytical ultracentrifugation

J Pearson, J Walter, W Peukert, H Cölfen - Analytical chemistry, 2018 - ACS Publications
This work highlights significant advancements in detector hardware and software for
multiwavelength analytical ultracentrifugation (MWL-AUC) experiments, demonstrating …

[HTML][HTML] Analytical ultracentrifugation in colloid and polymer science: new possibilities and perspectives after 100 years

H Cölfen - Colloid and Polymer Science, 2023 - Springer
Analytical ultracentrifugation (AUC) is a classical polymer and colloid analysis technique
invented by Theodor Svedberg 100 years ago. Modern hard-and software and powerful …

[HTML][HTML] Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment

A Savelyev, GE Gorbet, A Henrickson… - PLoS computational …, 2020 - journals.plos.org
Recent advances in instrumentation have moved analytical ultracentrifugation (AUC) closer
to a possible validation in a Good Manufacturing Practices (GMP) environment. In order for …

[HTML][HTML] Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

CR Horne, H Venugopal, S Panjikar, DM Wood… - Nature …, 2021 - nature.com
Bacteria respond to environmental changes by inducing transcription of some genes and
repressing others. Sialic acids, which coat human cell surfaces, are a nutrient source for …

Analysis of magic-size clusters in crude reaction mixtures using multiwavelength analytical ultracentrifugation

E Peters, R Rosenberg, H Cölfen… - The Journal of Physical …, 2022 - ACS Publications
Magic-size clusters are ultrasmall, transient species that appear early during semiconductor
nanocrystal growth and have been assigned to stoichiometrically and geometrically well …

Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments

B Demeler - Current Protocols, 2024 - Wiley Online Library
Analytical ultracentrifugation experiments play an integral role in the solution‐phase
characterization of biological macromolecules and their interactions. This unit discusses the …