A critical review: recent advancements in the use of CRISPR/Cas9 technology to enhance crops and alleviate global food crises

A Rasheed, RA Gill, MU Hassan, A Mahmood… - Current Issues in …, 2021 - mdpi.com
Genome editing (GE) has revolutionized the biological sciences by creating a novel
approach for manipulating the genomes of living organisms. Many tools have been …

Modern trends in plant genome editing: an inclusive review of the CRISPR/Cas9 toolbox

A Razzaq, F Saleem, M Kanwal, G Mustafa… - International journal of …, 2019 - mdpi.com
Increasing agricultural productivity via modern breeding strategies is of prime interest to
attain global food security. An array of biotic and abiotic stressors affect productivity as well …

Genome editing—principles and applications for functional genomics research and crop improvement

H Zhang, J Zhang, Z Lang, JR Botella… - Critical Reviews in Plant …, 2017 - Taylor & Francis
Genome editing technologies are powerful tools for studying gene function and for crop
improvement. The technologies rely on engineered endonucleases to generate double …

Diatom frustule morphogenesis and function: a multidisciplinary survey

E De Tommasi, J Gielis, A Rogato - Marine genomics, 2017 - Elsevier
Diatoms represent the major component of phytoplankton and are responsible for about 20–
25% of global primary production. Hundreds of millions of years of evolution led to tens of …

Current bioinformatics tools to optimize CRISPR/Cas9 experiments to reduce off-target effects

M Naeem, OS Alkhnbashi - International Journal of Molecular Sciences, 2023 - mdpi.com
The CRISPR-Cas system has evolved into a cutting-edge technology that has transformed
the field of biological sciences through precise genetic manipulation. CRISPR/Cas9 …

Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

Y You, X Sun, M Ma, J He, L Li, FW Porto… - Nature …, 2022 - nature.com
Trypsin is best known as a digestive enzyme in animals, but remains unexplored in
phytoplankton, the major primary producers in the ocean. Here we report the prevalence of …

CRISPR/Cas9: an RNA‐guided highly precise synthetic tool for plant genome editing

Y Demirci, B Zhang, T Unver - Journal of cellular physiology, 2018 - Wiley Online Library
CRISPR/Cas9 is a newly developed and naturally occurred genome editing tool, which is
originally used by bacteria for immune defence. In the past years, it has been quickly …

Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid

RG Dorrell, Y Zhang, Y Liang, N Gueguen… - The Plant …, 2024 - academic.oup.com
Organic carbon fixed in chloroplasts through the Calvin–Benson–Bassham Cycle can be
diverted toward different metabolic fates, including cytoplasmic and mitochondrial …

Design of guide RNA for CRISPR/Cas plant genome editing

GA Gerashchenkov, NA Rozhnova, BR Kuluev… - Molecular Biology, 2020 - Springer
CRISPR/Cas technology of genome editing is a powerful tool for making targeted changes
in the DNA of various organisms, including plants. The choice of the precise nucleotide …

Multiplexed Knockouts in the Model Diatom Phaeodactylum by Episomal Delivery of a Selectable Cas9

MA Moosburner, P Gholami, JK McCarthy… - Frontiers in …, 2020 - frontiersin.org
Marine diatoms are eukaryotic microalgae that play significant ecological and
biogeochemical roles in oceans. They also have significant potential as organismal …