Zebrafish have several advantages compared to other vertebrate models used in modeling
human diseases, particularly for large-scale genetic mutant and therapeutic compound …

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present
the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni …

Adenine base editors (ABEs) composed of an engineered adenine deaminase and the
Streptococcus pyogenes Cas9 nickase enable adenine-to-guanine (A-to-G) single …

Symbionts play an indispensable role in gut homeostasis, but underlying mechanisms
remain elusive. To clarify the role of lactic-acid-producing bacteria (LAB) on intestinal stem …

The combined availability of whole genome sequences and genome editing tools is set to
revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic …

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …

T-cell genome engineering holds great promise for cell-based therapies for cancer, HIV,
primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human …

CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are
enabling innovative applications of mammalian cell engineering. However, the delivery of …