[HTML][HTML] Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production

R Mans, JMG Daran, JT Pronk - Current opinion in biotechnology, 2018 - Elsevier
Highlights•Recent technological advances amplify the power of yeast evolutionary
engineering.•Dynamic selection regimes can mitigate phenotypic trade-off …

Biodefense in the age of synthetic biology

National Academies of Sciences, Division on Earth… - 2018 - books.google.com
Scientific advances over the past several decades have accelerated the ability to engineer
existing organisms and to potentially create novel ones not found in nature. Synthetic …

Creating a functional single-chromosome yeast

Y Shao, N Lu, Z Wu, C Cai, S Wang, LL Zhang, F Zhou… - Nature, 2018 - nature.com
Eukaryotic genomes are generally organized in multiple chromosomes. Here we have
created a functional single-chromosome yeast from a Saccharomyces cerevisiae haploid …

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Y Shen, Y Wang, T Chen, F Gao, J Gong, D Abramczyk… - Science, 2017 - science.org
INTRODUCTION Although much effort has been devoted to studying yeast in the past few
decades, our understanding of this model organism is still limited. Rapidly developing DNA …

Engineering the ribosomal DNA in a megabase synthetic chromosome

W Zhang, G Zhao, Z Luo, Y Lin, L Wang, Y Guo… - Science, 2017 - science.org
INTRODUCTION It has long been an interesting question whether a living cell can be
constructed from scratch in the lab, a goal that may not be realized anytime soon …

Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions

Y Zhao, C Coelho, AL Hughes, L Lazar-Stefanita… - Cell, 2023 - cell.com
The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

Precise control of SCRaMbLE in synthetic haploid and diploid yeast

B Jia, Y Wu, BZ Li, LA Mitchell, H Liu, S Pan… - Nature …, 2018 - nature.com
Compatibility between host cells and heterologous pathways is a challenge for constructing
organisms with high productivity or gain of function. Designer yeast cells incorporating the …

Design, construction, and functional characterization of a tRNA neochromosome in yeast

D Schindler, RSK Walker, S Jiang, AN Brooks, Y Wang… - Cell, 2023 - cell.com
Here, we report the design, construction, and characterization of a tRNA neochromosome, a
designer chromosome that functions as an additional, de novo counterpart to the native …

Enzymatic DNA synthesis by engineering terminal deoxynucleotidyl transferase

X Lu, J Li, C Li, Q Lou, K Peng, B Cai, Y Liu, Y Yao… - ACS …, 2022 - ACS Publications
Template-free enzymatic approaches are considered the most promising solution for next-
generation artificial DNA synthesis. However, the development of these technologies has …

Building a eukaryotic chromosome arm by de novo design and synthesis

S Jiang, Z Luo, J Wu, K Yu, S Zhao, Z Cai, W Yu… - Nature …, 2023 - nature.com
The genome of an organism is inherited from its ancestor and continues to evolve over time,
however, the extent to which the current version could be altered remains unknown. To …