CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …
Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
C Xu, Y Zhou, Q Xiao, B He, G Geng, Z Wang, B Cao… - Nature …, 2021 - nature.com
Competitive coevolution between microbes and viruses has led to the diversification of
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …
High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects
CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …
Massively parallel Cas13 screens reveal principles for guide RNA design
Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable
specific and robust target gene knockdown without altering the genome. To define rules for …
specific and robust target gene knockdown without altering the genome. To define rules for …
CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells
CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for
detecting and depleting RNAs of interest. Near perfect complementarity between a target …
detecting and depleting RNAs of interest. Near perfect complementarity between a target …
Structural basis for the activation of a compact CRISPR-Cas13 nuclease
The CRISPR-Cas13 ribonucleases have been widely applied for RNA knockdown and
transcriptional modulation owing to their high programmability and specificity. However, the …
transcriptional modulation owing to their high programmability and specificity. However, the …
Collateral activity of the CRISPR/RfxCas13d system in human cells
CRISPR/Cas13 systems are increasingly used for programmable targeting of RNAs. While
Cas13 nucleases are capable of degrading both target RNAs and bystander RNAs in vitro …
Cas13 nucleases are capable of degrading both target RNAs and bystander RNAs in vitro …
Applications of CRISPR/Cas13-based RNA editing in plants
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-
associated (Cas) system is widely used as a genome-editing tool in various organisms …
associated (Cas) system is widely used as a genome-editing tool in various organisms …
[HTML][HTML] The double life of CRISPR–Cas13
JF Bot, J van der Oost, N Geijsen - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•CRISPR–Cas13 is potentially capable of collateral RNA cleavage in eukaryotic
cells.•Target RNA levels, cell size, and cell-type-specific properties may influence the …
cells.•Target RNA levels, cell size, and cell-type-specific properties may influence the …