Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …

Competitive coevolution between microbes and viruses has led to the diversification of
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …

CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …

Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable
specific and robust target gene knockdown without altering the genome. To define rules for …

CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for
detecting and depleting RNAs of interest. Near perfect complementarity between a target …

The CRISPR-Cas13 ribonucleases have been widely applied for RNA knockdown and
transcriptional modulation owing to their high programmability and specificity. However, the …

CRISPR/Cas13 systems are increasingly used for programmable targeting of RNAs. While
Cas13 nucleases are capable of degrading both target RNAs and bystander RNAs in vitro …

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-
associated (Cas) system is widely used as a genome-editing tool in various organisms …

Highlights•CRISPR–Cas13 is potentially capable of collateral RNA cleavage in eukaryotic
cells.•Target RNA levels, cell size, and cell-type-specific properties may influence the …