During adaptive immunity, B cells differentiate either into memory B cells or plasma cells and
produce antibodies against foreign antigens to fight infection. Additionally, they behave as …

Large-scale sequencing of RNA from individual cells can reveal patterns of gene, isoform
and allelic expression across cell types and states. However, current short-read single-cell …

The immune mechanisms that cause tissue injury in lupus nephritis have been challenging
to define. The advent of high-dimensional cellular analyses, such as single-cell RNA …

High-throughput short-read sequencing has revolutionized how transcriptomes are
quantified and annotated. However, while Illumina short-read sequencers can be used to …

Despite the rapid advance in single-cell RNA sequencing (scRNA-seq) technologies within
the last decade, single-cell transcriptome analysis workflows have primarily used gene …

The development of high-throughput sequencing (next-generation sequencing technology
(NGS)) and the continuous increase in experimental throughput require the upstream …

Diverse RNA 5′ ends are generated through both transcriptional and post-transcriptional
processes. These important modes of gene regulation often vary across cell types and can …

Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-
throughput sequencing applications. Here, we report that the Tn5 transposase is also …

Next-generation sequencing approaches have fundamentally changed the types of
questions that can be asked about gene function and regulation. With the goal of …

Hematopoiesis is arguably one of the best understood stem cell systems; however,
significant challenges remain to reach a consensus understanding of the lineage potential …