Gene expression is regulated at multiple levels, and cells need to integrate and coordinate
different layers of control to implement the information in the genome. Post-transcriptional …

Several studies have shown that promoters of protein-coding genes are origins of pervasive
non-coding RNA transcription and can initiate transcription in both directions. However, only …

We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant,
PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription …

Deep sequencing now provides detailed snapshots of ribosome occupancy on mRNAs. We
leverage these data to parameterize a computational model of translation, keeping track of …

Cellular RNA levels are determined by the interplay of RNA production, processing and
degradation. However, because most studies of RNA regulation do not distinguish the …

Philosopher, sociologist and urban theorist, Henri Lefebvre is one of the great social
theorists of the twentieth century. This accessible and innovative introduction to the work of …

Maintaining proper mRNA levels is a key aspect in the regulation of gene expression. The
balance between mRNA synthesis and decay determines these levels. We demonstrate that …

Degradation of mRNA is one of the key processes that control the steady-state level of gene
expression. However, the rate of mRNA decay for the majority of genes is not known. We …

Noise in gene expression is generated at multiple levels, such as transcription and
translation, chromatin remodeling and pathway-specific regulation. Studies of individual …

To obtain rates of mRNA synthesis and decay in yeast, we established dynamic
transcriptome analysis (DTA). DTA combines non‐perturbing metabolic RNA labeling with …