Cutting back malaria: CRISPR/Cas9 genome editing of Plasmodium

MCS Lee, SE Lindner, JJ Lopez-Rubio… - Briefings in functional …, 2019 - academic.oup.com
CRISPR/Cas9 approaches are revolutionizing our ability to perform functional genomics
across a wide range of organisms, including the Plasmodium parasites that cause malaria …

Malaria parasite translocon structure and mechanism of effector export

CM Ho, JR Beck, M Lai, Y Cui, DE Goldberg, PF Egea… - Nature, 2018 - nature.com
The putative Plasmodium translocon of exported proteins (PTEX) is essential for transport of
malarial effector proteins across a parasite-encasing vacuolar membrane into host …

Label-free target protein characterization for small molecule drugs: recent advances in methods and applications

F Feng, W Zhang, Y Chai, D Guo, X Chen - Journal of Pharmaceutical and …, 2023 - Elsevier
Target protein identification is the key to identification of the mechanisms, side effects, and
evaluating druglikeness of small-molecule drugs. The commonly used “labeled” target …

Plasmepsins IX and X are essential and druggable mediators of malaria parasite egress and invasion

AS Nasamu, S Glushakova, I Russo, B Vaupel… - Science, 2017 - science.org
Proteases of the malaria parasite Plasmodium falciparum have long been investigated as
drug targets. The P. falciparum genome encodes 10 aspartic proteases called plasmepsins …

A PPP-type pseudophosphatase is required for the maintenance of basal complex integrity in Plasmodium falciparum

AA Morano, RM Rudlaff, JD Dvorin - Nature Communications, 2023 - nature.com
During its asexual blood stage, P. falciparum replicates via schizogony, wherein dozens of
daughter cells are formed within a single parent. The basal complex, a contractile ring that …

EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX

M Garten, AS Nasamu, JC Niles, J Zimmerberg… - Nature …, 2018 - nature.com
Intraerythrocytic malaria parasites reside within a parasitophorous vacuolar membrane
(PVM) generated during host cell invasion. Erythrocyte remodelling and parasite metabolism …

An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes

S Batinovic, E McHugh, SA Chisholm… - Nature …, 2017 - nature.com
The malaria parasite, Plasmodium falciparum, displays the P. falciparum erythrocyte
membrane protein 1 (Pf EMP1) on the surface of infected red blood cells (RBCs). We here …

Critical role for isoprenoids in apicoplast biogenesis by malaria parasites

M Okada, K Rajaram, RP Swift, A Mixon, JA Maschek… - Elife, 2022 - elifesciences.org
Isopentenyl pyrophosphate (IPP) is an essential metabolic output of the apicoplast organelle
in Plasmodium falciparum malaria parasites and is required for prenylation-dependent …

Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis

ES Istvan, S Das, S Bhatnagar, JR Beck, E Owen… - Elife, 2019 - elifesciences.org
Plasmodium parasites possess a protein with homology to Niemann-Pick Type C1 proteins
(Niemann-Pick Type C1-Related protein, NCR1). We isolated parasites with resistance …

Deletion of the Plasmodium falciparum exported protein PTP7 leads to Maurer's clefts vesiculation, host cell remodeling defects, and loss of surface presentation of …

OMS Carmo, GJ Shami, D Cox, B Liu, AJ Blanch… - Plos …, 2022 - journals.plos.org
Presentation of the variant antigen, Plasmodium falciparum erythrocyte membrane protein 1
(EMP1), at knob-like protrusions on the surface of infected red blood cells, underpins the …