Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Magnify is a universal molecular anchoring strategy for expansion microscopy

A Klimas, BR Gallagher, P Wijesekara, S Fekir… - Nature …, 2023 - nature.com
Expansion microscopy enables nanoimaging with conventional microscopes by physically
and isotropically magnifying preserved biological specimens embedded in a crosslinked …

Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy

D Sarkar, J Kang, AT Wassie, ME Schroeder… - Nature biomedical …, 2022 - nature.com
Many crowded biomolecular structures in cells and tissues are inaccessible to labelling
antibodies. To understand how proteins within these structures are arranged with nanoscale …

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)

HGJ Damstra, B Mohar, M Eddison, A Akhmanova… - Elife, 2022 - elifesciences.org
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light
microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in …

Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier

DA Helmerich, G Beliu, D Taban, M Meub, M Streit… - Nature …, 2022 - nature.com
Advances in super-resolution microscopy have demonstrated single-molecule localization
precisions of a few nanometers. However, translation of such high localization precisions …

iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

V Louvel, R Haase, O Mercey, MH Laporte… - Nature …, 2023 - nature.com
Expansion microscopy (ExM) is a highly effective technique for super-resolution
fluorescence microscopy that enables imaging of biological samples beyond the diffraction …

Technological advances in super-resolution microscopy to study cellular processes

C Bond, AN Santiago-Ruiz, Q Tang, M Lakadamyali - Molecular Cell, 2022 - cell.com
Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Advanced imaging and labelling methods to decipher brain cell organization and function

D Choquet, M Sainlos, JB Sibarita - Nature Reviews Neuroscience, 2021 - nature.com
The brain is arguably the most complex organ. The branched and extended morphology of
nerve cells, their subcellular complexity, the multiplicity of brain cell types as well as their …