Biomolecular condensates are dense assemblies of proteins that form distinct biochemical
compartments without being surrounded by a membrane. Some, such as P granules and …

Single-molecule fluorescence resonance energy transfer (smFRET) is a useful tool for
observing the dynamics of protein–nucleic acid interactions. Although most smFRET …

Transcription factors (TFs) regulate gene expression by binding DNA sequences recognized
by their DNA-binding domains (DBDs). DBD-recognized motifs are short and highly …

Despite their diverse biochemical characteristics and functions, all DNA-binding proteins
share the ability to accurately locate their target sites among the vast excess of non-target …

Sequence-specific binding of proteins to DNA is essential for accessing genetic information.
We derive a model that predicts an anticorrelation between the macroscopic association and …

Highlights•Transcription is controlled by large numbers of factors. Mechanistic models
require quantitative approaches.•Single Molecule Tracking provides direct measurements of …

Replication origins are licensed by loading two Mcm2-7 helicases around DNA in a head-to-
head conformation poised to initiate bidirectional replication. This process requires origin …

To predict transcription, one needs a mechanistic understanding of how the numerous
required transcription factors (TFs) explore the nuclear space to find their target genes …

Localization of single fluorescent molecules is key for physicochemical and biophysical
measurements, such as single-molecule tracking and super-resolution imaging by single …

Reaching sub-millisecond 3D tracking of individual molecules in living cells would enable
direct measurements of diffusion-limited macromolecular interactions under physiological …