Mammalian genomes express two principal gene categories through RNA polymerase II-
mediated transcription: protein-coding transcription units and non-coding RNA transcription …

The eukaryotic transcription apparatus synthesizes a staggering diversity of RNA molecules.
The labour of nuclear gene transcription is, therefore, divided among multiple DNA …

Summary RNA polymerase II (RNAPII) pausing in early elongation is critical for gene
regulation. Paused RNAPII can be released into productive elongation by the kinase P-TEFb …

Integrator is a metazoan-specific protein complex capable of inducing termination at all
RNAPII-transcribed loci. Integrator recognizes paused, promoter-proximal RNAPII and …

In higher eukaryotes, fine-tuned activation of protein-coding genes and many non-coding
RNAs pivots around the regulated activity of RNA polymerase II (Pol II). The Integrator …

Formation of the 3′ end of a eukaryotic mRNA is a key step in the production of a mature
transcript. This process is mediated by a number of protein factors that cleave the pre …

The elongation stage of transcription by RNA polymerase II (RNA Pol II) is central to the
regulation of gene expression in response to developmental and environmental cues in …

Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is
conserved among metazoa. Here, we report three cryo-EM structures of human Elongin …

A central goal of genetics is to define the relationships between genotypes and phenotypes.
High-content phenotypic screens such as Perturb-seq (CRISPR-based screens with single …

The pause-release model of transcription proposes that 40–100 bases from the start site
RNA Pol II pauses, followed by release into productive elongation. Pause release is …