Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Super-resolution microscopy depends on steps that can contribute to the formation of image
artifacts, leading to misinterpretation of biological information. We present NanoJ …

High-density analysis methods for localization microscopy increase acquisition speed but
produce artifacts. We demonstrate that these artifacts can be eliminated by the combination …

Heterogeneity in cell populations is a phenomenon encountered in virtually all biological
systems, in whole tissues and cell cultures and from bacterial to mammalian cells. Moreover …

Traditional approaches for finding well-performing parameterizations of complex imaging
systems, such as super-resolution microscopes rely on an extensive exploration phase over …

Background Fusion of DNA methyltransferase domains to the nuclease-deficient clustered
regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has …

Methods that fuse multiple localization microscopy images of a single structure can improve
signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity …

Super-resolution light microscopy (SRM) offers a unique opportunity for diffraction-unlimited
imaging of biomolecular activities in living cells. To realize such potential, genetically …