A compendium of single extracellular vesicle flow cytometry

JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …

Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

KP De Sousa, I Rossi, M Abdullahi… - Wiley …, 2023 - Wiley Online Library
Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer
nanoparticles secreted by most cells. EVs are regarded as important mediators of …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

A versatile platform for generating engineered extracellular vesicles with defined therapeutic properties

K Dooley, RE McConnell, K Xu, ND Lewis, S Haupt… - Molecular Therapy, 2021 - cell.com
Extracellular vesicles (EVs) are an important intercellular communication system facilitating
the transfer of macromolecules between cells. Delivery of exogenous cargo tethered to the …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Characterizing extracellular vesicles and their diverse RNA contents

EM Veziroglu, GI Mias - Frontiers in Genetics, 2020 - frontiersin.org
Cells release nanometer-scale, lipid bilayer-enclosed biomolecular packages (extracellular
vesicles; EVs) into their surrounding environment. EVs are hypothesized to be intercellular …

Methodological guidelines to study extracellular vesicles

FAW Coumans, AR Brisson, EI Buzas… - Circulation …, 2017 - Am Heart Assoc
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …

Analysis of extracellular vesicle DNA at the single‐vesicle level by nano‐flow cytometry

H Liu, Y Tian, C Xue, Q Niu, C Chen… - Journal of extracellular …, 2022 - Wiley Online Library
It has been demonstrated recently that extracellular vesicles (EVs) carry DNA; however,
many fundamental features of DNA in EVs (EV‐DNA) remain elusive. In this study, a …

Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry

Y Tian, M Gong, Y Hu, H Liu, W Zhang… - Journal of …, 2020 - Taylor & Francis
Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent
potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with …

Protein Profiling and Sizing of Extracellular Vesicles from Colorectal Cancer Patients via Flow Cytometry

Y Tian, L Ma, M Gong, G Su, S Zhu, W Zhang, S Wang… - ACS …, 2018 - ACS Publications
Extracellular vesicles (EVs) have stimulated considerable scientific and clinical interest, yet
protein profiling and sizing of individual EVs remains challenging due to their small particle …