Stable production of value-added products using a microbial chassis is pivotal for
determining the industrial suitability of the engineered biocatalyst. Microbial cells often lose …

Bacteria are a treasure trove of metabolic reactions, but most industrial biotechnology
applications rely on a limited set of established host organisms. In contrast, adopting …

Exploiting biological processes to recycle renewable carbon into high value platform
chemicals provides a sustainable and greener alternative to current reliance on …

Hydroxypropionate (3-HP) is a versatile compound for chemical synthesis and a potential
building block for biodegradable polymers. Cupriavidus necator H16, a facultative …

It is impractical to develop a new parts collection for every potential host organism. It is well-
established that gene expression parts, like genes, are qualitatively transferable, but there is …

Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including
chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene …

Background Existing plasmid systems offer a fundamental foundation for gene expression in
Cupriavidus necator; however, their applicability is constrained by the limitations of …

Advanced genome engineering enables precise and customizable modifications of bacterial
species, and toolsets that exhibit broad-host compatibility are particularly valued owing to …

Polyhydroxyalkanoates are attractive alternatives to traditional plastics. However, although
polyhydroxybutyrate (PHB) is produced in large quantities by Cupriavidus necator H16, its …

Parageobacillus thermoglucosidasius is a promising chassis for producing chemicals and
fuels. Here we designed, built and tested the pMTL60000 modular plasmids containing …