Fundamental Aspects of Phase-Separated Biomolecular Condensates

HX Zhou, D Kota, S Qin, R Prasad - Chemical Reviews, 2024 - ACS Publications
Biomolecular condensates, formed through phase separation, are upending our
understanding in much of molecular, cell, and developmental biology. There is an urgent …

Biomolecular condensates in contact with membranes

A Mangiarotti, R Dimova - Annual Review of Biophysics, 2024 - annualreviews.org
Biomolecular condensates are highly versatile membraneless organelles involved in a
plethora of cellular processes. Recent years have witnessed growing evidence of the …

Liquid spherical shells are a non-equilibrium steady state of active droplets

AM Bergmann, J Bauermann, G Bartolucci… - Nature …, 2023 - nature.com
Liquid-liquid phase separation yields spherical droplets that eventually coarsen to one
large, stable droplet governed by the principle of minimal free energy. In chemically fueled …

Thermodynamic forces from protein and water govern condensate formation of an intrinsically disordered protein domain

S Mukherjee, LV Schäfer - Nature Communications, 2023 - nature.com
Liquid-liquid phase separation (LLPS) can drive a multitude of cellular processes by
compartmentalizing biological cells via the formation of dense liquid biomolecular …

Multiphasic coacervates assembled by hydrogen bonding and hydrophobic interactions

X Liu, AH Mokarizadeh, A Narayanan… - Journal of the …, 2023 - ACS Publications
Coacervation has emerged as a prevalent mechanism to compartmentalize biomolecules in
living cells. Synthetic coacervates help in understanding the assembly process and mimic …

Biomolecular condensates modulate membrane lipid packing and hydration

A Mangiarotti, M Siri, NW Tam, Z Zhao… - Nature …, 2023 - nature.com
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in
cell biology, playing an important role in a diverse range of processes across different …

Visualizing formation and dynamics of a three-dimensional sponge-like network of a coacervate in real time

R Kubota, T Hiroi, Y Ikuta, Y Liu… - Journal of the American …, 2023 - ACS Publications
Coacervates, which are formed by liquid–liquid phase separation, have been extensively
explored as models for synthetic cells and membraneless organelles, so their in-depth …

Dominance analysis to assess solute contributions to multicomponent phase equilibria

D Qian, H Ausserwoger, T Sneideris, M Farag… - Proceedings of the …, 2024 - pnas.org
Phase separation in aqueous solutions of macromolecules underlies the generation of
biomolecular condensates in cells. Condensates are membraneless bodies, representing …

Femtoliter oil droplets act as CO2 micropumps for uninterrupted electrochemiluminescence at the water| oil interface

S Voci, KJ Vannoy, JE Dick - Journal of Colloid and Interface Science, 2024 - Elsevier
Interfacial effects are well-known to significantly alter chemical reactivity, especially in
confined environments, where the surface to volume ratio increases. Here, we observed an …

Protein Compartments Modulate Fibrillar Self‐Assembly

S Karger, ME Miali, A Solomonov, D Eliaz, N Varsano… - Small, 2024 - Wiley Online Library
A notable feature of complex cellular environments is protein‐rich compartments that are
formed via liquid–liquid phase separation. Recent studies have shown that these …