Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize
these events without interference from other regions deeper within. This review describes a …

▪ Abstract Membrane fusion involves the merger of two phospholipid bilayers in an aqueous
environment. In artificial lipid bilayers, fusion proceeds by means of defined transition states …

Synapse,” intended to contrast our grasp of presynaptic Angleson and Betz (1997) quite
recently reviewed in physiology with our understanding of postsynaptic detail techniques for …

Regulated exocytosis of secretory granules or dense-core granules has been examined in
many well-characterized cell types including neurons, neuroendocrine, endocrine, exocrine …

Owing to the increasing interest in self-assembled structures from block copolymer
materials, we present here a review of recent literature concerning amphiphilic block …

Publisher Summary Total internal reflection fluorescence (TIRF) provides a means to
selectively excite just those fluorophores in an aqueous environment that are very near a …

To sustain high rates of transmitter release, synaptic terminals must rapidly re-supply
vesicles to release sites and prime them for exocytosis. Here we describe imaging of single …

The plasma membrane is a two-dimensional compartment that relays most biological
signals sent or received by a cell. Signalling involves membrane receptors and their …

Publisher Summary This chapter describes the total internal reflection fluorescence (TIRF)
microscopy in cell biology. TIRF microscopy (also called “evanescent wave microscopy”) …

Evanescent wave microscopy, also termed total internal reflection fluorescence microscopy
(TIR-FM), has shed new light on important cellular processes taking place near the plasma …