The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

Genetically engineered mouse models are commonly preferred for studying the human
disease due to genetic and pathophysiological similarities between mice and humans. In …

A technique for chromosomal insertion of large DNA segments is much needed in plant
breeding and synthetic biology to facilitate the introduction of desired agronomic traits and …

Insertion sequence (IS) elements are the simplest autonomous transposable elements found
in prokaryotic genomes. We recently discovered that IS110 family elements encode a …

All cellular life forms are afflicted by diverse genetic parasites, including viruses and other
types of mobile genetic elements (MGEs), and have evolved multiple, diverse defence …

Red light penetrates deep into mammalian tissues and has low phototoxicity, but few
optogenetic tools that use red light have been developed. Here we present MagRed, a red …

Genome engineering techniques represented by the Cre–loxP recombination system have
been used extensively for biomedical research. However, powerful and useful techniques …

The steady progress in genome editing, especially genome editing based on the use of
clustered regularly interspaced short palindromic repeats (CRISPR) and programmable …

Microbes are increasingly employed as cell factories to produce biomolecules. This often
involves the expression of complex heterologous biosynthesis pathways in host strains …

Cell line development represents a crucial step in the development process of a therapeutic
glycoprotein. Chinese hamster ovary (CHO) cells are the most frequently employed …