Ranking the biases: The choice of OTUs vs. ASVs in 16S rRNA amplicon data analysis has stronger effects on diversity measures than rarefaction and OTU identity …

M Chiarello, M McCauley, S Villéger, CR Jackson - PloS one, 2022 - journals.plos.org
Advances in the analysis of amplicon sequence datasets have introduced a methodological
shift in how research teams investigate microbial biodiversity, away from sequence identity …

Nanopore is preferable over Illumina for 16S amplicon sequencing of the gut microbiota when species-level taxonomic classification, accurate estimation of richness …

M Szoboszlay, L Schramm, D Pinzauti, J Scerri… - Microorganisms, 2023 - mdpi.com
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon
sequencing as it can provide full-length 16S reads and has a low up-front cost that allows …

Microbial community and geochemical analyses of trans-trench sediments for understanding the roles of hadal environments

S Hiraoka, M Hirai, Y Matsui, A Makabe… - The ISME …, 2020 - academic.oup.com
Hadal trench bottom (> 6000 m below sea level) sediments harbor higher microbial cell
abundance compared with adjacent abyssal plain sediments. This is supported by the …

Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling

S Reitmeier, TCA Hitch, N Treichel, N Fikas… - ISME …, 2021 - academic.oup.com
Abstract 16S rRNA gene amplicon sequencing is a popular approach for studying
microbiomes. However, some basic concepts have still not been investigated …

Deciphering intra-species bacterial diversity of meat and seafood spoilage microbiota using gyrB amplicon sequencing: A comparative analysis with 16S rDNA V3-V4 …

S Poirier, O Rué, R Peguilhan, G Coeuret, M Zagorec… - PLoS …, 2018 - journals.plos.org
Meat and seafood spoilage ecosystems harbor extensive bacterial genomic diversity that is
mainly found within a small number of species but within a large number of strains with …

Microbiota composition and functional profiling throughout the gastrointestinal tract of commercial weaning piglets

R Gresse, F Chaucheyras Durand, L Dunière… - Microorganisms, 2019 - mdpi.com
Dietary, environmental, and social stresses induced by weaning transition in pig production
are associated with alterations of gut microbiota, diarrhea, and enteric infections. With the …

Uncovering the potential of termite gut microbiome for lignocellulose bioconversion in anaerobic batch bioreactors

L Auer, A Lazuka, D Sillam-Dussès, E Miambi… - Frontiers in …, 2017 - frontiersin.org
Termites are xylophages, being able to digest a wide variety of lignocellulosic biomass
including wood with high lignin content. This ability to feed on recalcitrant plant material is …

Current practice in plankton metabarcoding: optimization and error management

LF Santoferrara - Journal of Plankton Research, 2019 - academic.oup.com
High-throughput sequencing of a targeted genetic marker is being widely used to analyze
biodiversity across taxa and environments. Amid a multitude of exciting findings, scientists …

A longitudinal characterization of the Non-Cystic Fibrosis Bronchiectasis airway microbiome

TE Woo, R Lim, AA Heirali, N Acosta, HR Rabin… - Scientific reports, 2019 - nature.com
A diverse microbiota exists within the airways of individuals with non-cystic fibrosis
bronchiectasis (nCFB). How the lung microbiome evolves over time, and whether changes …

Improving eDNA‐based protist diversity assessments using networks of amplicon sequence variants

D Forster, G Lentendu, S Filker… - Environmental …, 2019 - Wiley Online Library
Effective and precise grouping of highly similar sequences remains a major bottleneck in the
evaluation of high‐throughput sequencing datasets. Amplicon sequence variants (ASVs) …