Mechanisms and consequences of macromolecular phase separation

LP Bergeron-Sandoval, N Safaee, SW Michnick - Cell, 2016 - cell.com
Over a century ago, colloidal phase separation of matter into non-membranous bodies was
recognized as a fundamental organizing principal of cell" protoplasm." Recent insights into …

A decade and a half of protein intrinsic disorder: biology still waits for physics

VN Uversky - Protein Science, 2013 - Wiley Online Library
The abundant existence of proteins and regions that possess specific functions without
being uniquely folded into unique 3D structures has become accepted by a significant …

[HTML][HTML] Nucleo-cytoplasmic partitioning of ARF proteins controls auxin responses in Arabidopsis thaliana

SK Powers, AS Holehouse, DA Korasick, KH Schreiber… - Molecular cell, 2019 - cell.com
The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and
development. The auxin response factor (ARF) transcription factor family regulates auxin …

Intrinsically disordered proteins from A to Z

VN Uversky - The international journal of biochemistry & cell biology, 2011 - Elsevier
The ideas that proteins might possess specific functions without being uniquely folded into
rigid 3D-structures and that these floppy polypeptides might constitute a noticeable part of …

Unmasking the roles of N-and C-terminal flanking sequences from exon 1 of huntingtin as modulators of polyglutamine aggregation

SL Crick, KM Ruff, K Garai… - Proceedings of the …, 2013 - National Acad Sciences
Huntington disease is caused by mutational expansion of the CAG trinucleotide within exon
1 of the huntingtin (Htt) gene. Exon 1 spanning N-terminal fragments (NTFs) of the Htt …

Essential role of coiled coils for aggregation and activity of Q/N-rich prions and PolyQ proteins

F Fiumara, L Fioriti, ER Kandel, WA Hendrickson - Cell, 2010 - cell.com
The functional switch of glutamine/asparagine (Q/N)-rich prions and the neurotoxicity of
polyQ-expanded proteins involve complex aggregation-prone structural transitions …

Connecting coil-to-globule transitions to full phase diagrams for intrinsically disordered proteins

X Zeng, AS Holehouse, A Chilkoti, T Mittag, RV Pappu - Biophysical journal, 2020 - cell.com
Phase separation is thought to underlie spatial and temporal organization that is required for
controlling biochemical reactions in cells. Multivalence of interaction motifs, also known as …

Proteins containing expanded polyglutamine tracts and neurodegenerative disease

A Adegbuyiro, F Sedighi, AW Pilkington IV… - Biochemistry, 2017 - ACS Publications
Several hereditary neurological and neuromuscular diseases are caused by an abnormal
expansion of trinucleotide repeats. To date, there have been 10 of these trinucleotide repeat …

Collapse transitions of proteins and the interplay among backbone, sidechain, and solvent interactions

AS Holehouse, RV Pappu - Annual Review of biophysics, 2018 - annualreviews.org
Proteins can collapse into compact globules or form expanded, solvent-accessible, coil-like
conformations. Additionally, they can fold into well-defined three-dimensional structures or …

Kinase inhibitors modulate huntingtin cell localization and toxicity

RS Atwal, CR Desmond, N Caron, T Maiuri… - Nature chemical …, 2011 - nature.com
Two serine residues within the first 17 amino acid residues of huntingtin (N17) are crucial for
modulation of mutant huntingtin toxicity in cell and mouse genetic models of Huntington's …