Development and future of droplet microfluidics

L Nan, H Zhang, DA Weitz, HC Shum - Lab on a Chip, 2024 - pubs.rsc.org
Over the past two decades, advances in droplet-based microfluidics have facilitated new
approaches to process and analyze samples with unprecedented levels of precision and …

Droplet-based microfluidics: applications in pharmaceuticals

TND Trinh, HDK Do, NN Nam, TT Dan, KTL Trinh… - Pharmaceuticals, 2023 - mdpi.com
Droplet-based microfluidics offer great opportunities for applications in various fields, such
as diagnostics, food sciences, and drug discovery. A droplet provides an isolated …

Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies

J Jiang, G Yang, F Ma - Biotechnology advances, 2023 - Elsevier
Fluorescence-activated droplet sorting (FADS) has emerged as a powerful tool for ultrahigh-
throughput screening of enzymes, metabolites, and antibodies. Fluorescence coupling …

微液滴高通量筛选方法的研究与应用进展

秦伟彤, 杨广宇 - 合成生物学, 2023 - synbioj.cip.com.cn
在单细胞层面对生物功能进行高通量的分析和分选是对关键基因, 元件, 途径与细胞工厂进行
优化的重要技术. 基于微液滴的筛选方法因其低成本, 超高通量等优势, 已被广泛应用于生物 …

Advanced strategies in high-throughput droplet screening for enzyme engineering

W Yan, X Li, D Zhao, M Xie, T Li, L Qian, C Ye… - Biosensors and …, 2023 - Elsevier
Enzymes, as biocatalysts, play a cumulatively important role in environmental purification
and industrial production of chemicals and pharmaceuticals. However, natural enzymes are …

Advances in ultrahigh-throughput screening technologies for protein evolution

Z Liu, S Chen, J Wu - Trends in Biotechnology, 2023 - cell.com
Inspired by natural evolution, directed evolution randomly mutates the gene of interest
through artificial evolution conditions with variants being screened for the required …

基于荧光检测的高通量筛选技术和装备助力细胞工厂构建

孙梦楚, 陆亮宇, 申晓林, 孙新晓, 王佳, 袁其朋 - 合成生物学, 2023 - synbioj.cip.com.cn
微生物工业制造是以微生物细胞工厂为核心, 利用低成本, 可再生资源为原料,
实现高附加值化合物的绿色生产. 依赖于“设计-构建-测试-学习”(DBTL) 循环的微生物细胞工厂 …

A capillary-based centrifugal indicator equipped with in situ pathogenic bacteria culture for fast antimicrobial susceptibility testing

L Chen, M Zhu, Z Wang, H Wang, Y Cheng, Z Zhang… - Analyst, 2024 - pubs.rsc.org
Antimicrobial resistance has become a major global health threat due to the misuse and
overuse of antibiotics. Rapid, affordable, and high-efficiency antimicrobial susceptibility …

Advances in Droplet-Based Microfluidic High-Throughput Screening of Engineered Strains and Enzymes Based on Ultraviolet, Visible, and Fluorescent Spectroscopy

S Hu, B Wang, Q Luo, R Zeng, J Zhang, J Cheng - Fermentation, 2023 - mdpi.com
Genetic engineering and directed evolution are effective methods for addressing the low
yield and poor industrialization level of microbial target products. The current research focus …

合成生物学与未来食品*

徐显皓, 刘龙, 陈坚 - 中国生物工程杂志, 2024 - manu60.magtech.com.cn
食品工业是保障国计民生的支柱产业. 随着人口增长, 环境污染, 气候变化等问题的日益突出,
亟需对传统的食品制造工艺进行转型升级. 合成生物学的发展为食品制造工艺的变革提供了技术 …