Advances and challenges in cell-free incorporation of unnatural amino acids into proteins

W Gao, E Cho, Y Liu, Y Lu - Frontiers in pharmacology, 2019 - frontiersin.org
Incorporation of unnatural amino acids (UNAAs) into proteins currently is an active biological
research area for various fundamental and applied science. In this context, cell-free …

Easy synthesis of complex biomolecular assemblies: wheat germ cell-free protein expression in structural biology

ML Fogeron, L Lecoq, L Cole, M Harbers… - Frontiers in Molecular …, 2021 - frontiersin.org
Cell-free protein synthesis (CFPS) systems are gaining more importance as universal tools
for basic research, applied sciences, and product development with new technologies …

Naturally acquired antibody responses to more than 300 Plasmodium vivax proteins in three geographic regions

RJ Longley, MT White, E Takashima… - PLoS neglected …, 2017 - journals.plos.org
Plasmodium vivax remains an important cause of malaria in South America and the Asia-
Pacific. Naturally acquired antibody responses against multiple P. vivax proteins have been …

Cell-free protein synthesis for producing 'difficult-to-express' proteins

X Jin, SH Hong - Biochemical Engineering Journal, 2018 - Elsevier
Biomanufacturing requires the stable production of active biological molecules including
proteins. However, a significant percentage of genes cannot be expressed efficiently in a …

KILchip v1.0: A Novel Plasmodium falciparum Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization

G Kamuyu, J Tuju, R Kimathi, K Mwai, J Mburu… - Frontiers in …, 2018 - frontiersin.org
Passive transfer studies in humans clearly demonstrated the protective role of IgG
antibodies against malaria. Identifying the precise parasite antigens that mediate immunity is …

[HTML][HTML] Leveraging the wheat germ cell-free protein synthesis system to accelerate malaria vaccine development

BN Kanoi, H Nagaoka, M Morita, T Tsuboi… - Parasitology …, 2021 - Elsevier
Vaccines against infectious diseases have had great successes in the history of public
health. Major breakthroughs have occurred in the development of vaccine-based …

Cell-free protein synthesis systems for vaccine design and production

VT Hu, NP Kamat - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•CFPS systems allow for highly optimizable and rapid protein production.•CFPS
overcomes limitations in current vaccine production and expands vaccine …

Identification of novel Plasmodium vivax proteins associated with protection against clinical malaria

R Mazhari, E Takashima, RJ Longley… - Frontiers in Cellular …, 2023 - frontiersin.org
As progress towards malaria elimination continues, the challenge posed by the parasite
species Plasmodium vivax has become more evident. In many regions co-endemic for P …

Antibody selection strategies and their impact in predicting clinical malaria based on multi-sera data

A Fonseca, M Spytek, P Biecek, C Cordeiro… - BioData Mining, 2024 - Springer
Background Nowadays, the chance of discovering the best antibody candidates for
predicting clinical malaria has notably increased due to the availability of multi-sera data …

Global Repertoire of Human Antibodies Against Plasmodium falciparum RIFINs, SURFINs, and STEVORs in a Malaria Exposed Population

BN Kanoi, H Nagaoka, MT White, M Morita… - Frontiers in …, 2020 - frontiersin.org
Clinical immunity to malaria develops after repeated exposure to Plasmodium falciparum
parasites. Broadly reactive antibodies against parasite antigens expressed on the surface of …