Highlights•Genetic improvement decreases the environmental footprint of animal-source
protein.•New breeding methods enable precise intraspecies and interspecies allele …

The increasing demand for recombinant vaccine antigens or immunotherapeutic molecules
calls into question the universality of current protein expression systems. Vaccine production …

The production of transgenic farm animals (eg, cattle) via genome engineering for the gain
or loss of gene functions is an important undertaking. In the initial stages of genome …

Sleeping Beauty (SB) is the first synthetic DNA transposon that was shown to be active in a
wide variety of species. Here, we review studies from the last two decades addressing both …

The recent establishment of induced pluripotent stem (iPS) cells promises the development
of autologous cell therapies for degenerative diseases, without the ethical concerns …

Background Gene transfer by electroporation is an established method for the non-viral
mediated transfection of mammalian cells. Primary cells pose a particular challenge for …

Genetically modified cattle are important for developing new biomedical models and for an
improved understanding of the pathophysiology of zoonotic diseases. However, genome …

Разработка технологий активного трансгенеза сделала возможным внесение
направленных изменений (геномное редактирование, genome editing, GE) в геном …

Since centuries, the traits for production and disease resistance are being targeted while
improving the genetic merit of domestic animals, using conventional breeding programs …

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and
somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm …