Protein production must be strictly controlled at its beginning and end to synthesize a
polypeptide that faithfully copies genetic information carried in the encoding mRNA. In …

Picobirnaviruses (PBVs) are bisegmented double-stranded RNA viruses that have been
detected in a wide variety of animal species including invertebrates and in environmental …

DAMBE is a comprehensive software workbench for data analysis in molecular biology,
phylogenetics, and evolution. Several important new functions have been added since …

The ribosome's interactions with mRNA govern its translation rate and the effects of post-
transcriptional regulation. Long, structured 5′ untranslated regions (5′ UTRs) are …

The pET series of expression plasmids are widely used for recombinant protein production
in Escherichia coli. The genetic modules controlling transcription and translation in these …

RNA-seq technologies have provided significant insight into the transcription networks of
mycobacteria. However, such studies provide no definitive information on the translational …

The use of alternative translation initiation sites enables production of more than one protein
from a single gene, thereby expanding the cellular proteome. Although several such …

Messenger RNA acts as an informational molecule between DNA and translating
ribosomes. Emerging evidence places mRNA in central cellular processes beyond its major …

Shine-Dalgarno (SD) motifs are thought to play an important role in translational initiation in
bacteria. Paradoxically, ribosome profiling studies in E. coli show no correlation between the …

Synthetic biology may be viewed as an effort to establish, formalize, and develop an
engineering discipline in the context of biological systems. The ability to tune the properties …