Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

In recent years, microbioreactor (MBR) systems have evolved towards versatile bioprocess
engineering tools. They provide a unique solution to combine higher experimental …

NMR spectroscopy is an essential analytical technique in metabolomics and fluxomics
workflows, owing to its high structural elucidation capabilities combined with its intrinsic …

White biotechnology is a term that is now often used to describe the implementation of
biotechnology in the industrial sphere. Biocatalysts (enzymes and microorganisms) are the …

Metabolic engineering involves the manipulation of microbes to produce desirable
compounds through genetic engineering or synthetic biology approaches. Metabolomics …

Highlights•Tracer experiments, analytics and modeling constitute the 13 C fluxomics
triumvirate.•13 C fluxomics tools differ in complexity, information power and universality.•The …

Metabolic flux analysis (MFA) quantitatively describes cellular fluxes to understand
metabolic phenotypes and functional behaviour after environmental and/or genetic …

Highlights•13 C-fluxomics provides quantification of metabolic activity in microorganisms.•
Increasing flux analysis capacity has the potential to accelerate strain design.•HT 13 C …

Highlights•Kinetic metabolic modeling could enable rational bioengineering and dynamic
understanding of metabolism.•We discuss experimental and computational approaches to …

Production of 3-hydroxypropionic acid (3-HP) in Pichia pastoris (syn. Komagataella phaffii)
via the malonyl-CoA pathway has been recently demonstrated using glycerol as a carbon …