Properties of dimeric, disulfide-linked rhBMP-2 recovered from E. coli derived inclusion bodies by mild extraction or chaotropic solubilization and subsequent refolding
B Quaas, L Burmeister, Z Li, M Nimtz, A Hoffmann… - Process …, 2018 - Elsevier
Recombinant human bone morphogenetic protein-2 (rhBMP-2), a cystine-knot containing
disulfide-linked homodimer, was produced in form of inclusion bodies (IBs) using E. coli …
disulfide-linked homodimer, was produced in form of inclusion bodies (IBs) using E. coli …
Organocatalysts of oxidative protein folding inspired by protein disulfide isomerase
JC Lukesh III, KA Andersen, KK Wallin… - Organic & biomolecular …, 2014 - pubs.rsc.org
Organocatalysts derived from diethylenetriamine effect the rapid isomerization of non-native
protein disulfide bonds to native ones. These catalysts contain a pendant hydrophobic …
protein disulfide bonds to native ones. These catalysts contain a pendant hydrophobic …
The dehydrogenase region of the NADPH oxidase component Nox2 acts as a protein disulfide isomerase (PDI) resembling PDIA3 with a role in the binding of the …
E Bechor, I Dahan, T Fradin, Y Berdichevsky… - Frontiers in …, 2015 - frontiersin.org
The superoxide (O·− 2)-generating NADPH oxidase of phagocytes consists of a membrane
component, cytochrome b 558 (a heterodimer of Nox2 and p22 phox), and four cytosolic …
component, cytochrome b 558 (a heterodimer of Nox2 and p22 phox), and four cytosolic …
Peptide disulfide RKCGCFF facilitates oxidative protein refolding by mimicking protein disulfide isomerase
H Liu, XY Dong, Y Sun - Biochemical engineering journal, 2013 - Elsevier
This communication reports a new design of peptide disulfide, RKCGCFF, for facilitating
oxidative protein refolding. The new design mimics the properties of protein disulfide …
oxidative protein refolding. The new design mimics the properties of protein disulfide …
Facilitated oxidative refolding of ribonuclease A from inclusion bodies with a new redox system
GJ Han, XY Dong, L Zhang, LT Fu, GZ Wang… - Biochemical engineering …, 2012 - Elsevier
Oxidative refolding is a crucial step in the bioseparation process of bioactive RNase A from
inclusion bodies (IBs) overexpressed in Escherichia coli, and it has been well recognized …
inclusion bodies (IBs) overexpressed in Escherichia coli, and it has been well recognized …
Organocatalysts of oxidative protein folding
RT Raines, JC LUKESH III - US Patent 9,630,916, 2017 - Google Patents
R3 R3 where R is hydrogen or—COR, where R is an optionally Substituted aliphatic group
or an optionally Substituted aryl group; R is hydrogen or—CO—Rs, where Rs is alkyl having …
or an optionally Substituted aryl group; R is hydrogen or—CO—Rs, where Rs is alkyl having …