[HTML][HTML] Process analytical technologies–advances in bioprocess integration and future perspectives

G Gerzon, Y Sheng, M Kirkitadze - Journal of pharmaceutical and …, 2022 - Elsevier
Abstract Process Analytical Technology (PAT) instruments include analyzers capable of
measuring physical and chemical process parameters and key attributes with the goal of …

[HTML][HTML] Protein glycosylation and glycoinformatics for novel biomarker discovery in neurodegenerative diseases

J Costa, C Hayes, F Lisacek - Ageing research reviews, 2023 - Elsevier
Glycosylation is a common post-translational modification of brain proteins including cell
surface adhesion molecules, synaptic proteins, receptors and channels, as well as …

Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies

M Alenquer, F Ferreira, D Lousa, M Valério… - PLoS …, 2021 - journals.plos.org
Understanding SARS-CoV-2 evolution and host immunity is critical to control COVID-19
pandemics. At the core is an arms-race between SARS-CoV-2 antibody and angiotensin …

A CHO stable pool production platform for rapid clinical development of trimeric SARS‐CoV‐2 spike subunit vaccine antigens

S Joubert, M Stuible, S Lord‐Dufour… - Biotechnology and …, 2023 - Wiley Online Library
Protein expression from stably transfected Chinese hamster ovary (CHO) clones is an
established but time‐consuming method for manufacturing therapeutic recombinant …

Population homogeneity for the antibody response to COVID-19 BNT162b2/Comirnaty vaccine is only reached after the second dose across all adult age ranges

J Faro-Viana, ML Bergman, LA Gonçalves… - Nature …, 2022 - nature.com
While mRNA vaccines are administrated worldwide in an effort to contain the COVID-19
pandemic, the heterogeneity of the humoral immune response they induce at the population …

Production of the SARS-CoV-2 spike protein and its receptor binding domain in plant cell suspension cultures

BA Rebelo, A Folgado, AC Ferreira… - Frontiers in Plant …, 2022 - frontiersin.org
The COVID-19 pandemic, caused by the worldwide spread of SARS-CoV-2, has prompted
the scientific community to rapidly develop efficient and specific diagnostics and …

Recombinant protein expression and purification of N, S1, and RBD of SARS-CoV-2 from mammalian cells and their potential applications

J Garcia-Cordero, J Mendoza-Ramirez… - Diagnostics, 2021 - mdpi.com
The coronavirus disease 2019 (COVID-19) pandemic has reached an unprecedented level.
There is a strong demand for diagnostic and serological supplies worldwide, making it …

Application of bioreactor technology for cell culture-based viral vaccine production: Present status and future prospects

Z Fang, J Lyu, J Li, C Li, Y Zhang, Y Guo… - … in Bioengineering and …, 2022 - frontiersin.org
Bioreactors are widely used in cell culture-based viral vaccine production, especially during
the coronavirus disease 2019 (COVID-19) pandemic. In this context, the development and …

Development of a scalable single process for producing SARS-CoV-2 RBD monomer and dimer vaccine antigens

T Boggiano-Ayo, J Palacios-Oliva… - … in Bioengineering and …, 2023 - frontiersin.org
We have developed a single process for producing two key COVID-19 vaccine antigens:
SARS-CoV-2 receptor binding domain (RBD) monomer and dimer. These antigens are …

Disposable rocking bioreactors: recent applications and progressive perspectives

K Wierzchowski, M Pilarek - Trends in Biotechnology, 2023 - cell.com
Disposable rocking bioreactors facilitate scaling up animal and plant cell biomass
propagation and developing specified bioprocesses like manufacturing vaccines or chimeric …