Microbial ecology as a scientific field is fundamentally driven by technological advance. The
past decade's revolution in DNA sequencing cost and throughput has made it possible for …

Novel high‐throughput sequencing methods outperform earlier approaches in terms of
resolution and magnitude. They enable identification and relative quantification of …

Environmental DNA (eDNA) refers to DNA that can be extracted from environmental
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …

The global diversity of Bacteria and Archaea, the most ancient and most widespread forms
of life on Earth, is a subject of intense controversy. This controversy stems largely from the …

Metabarcoding of DNA extracted from environmental or bulk specimen samples is
increasingly used to profile biota in basic and applied biodiversity research because of its …

The human body is home to a complex and diverse microbial ecosystem that plays a central
role in host health. This includes a diversity of fungal species that is collectively referred to …

Next generation metabarcoding is becoming an indispensable tool in fungal community
ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but …

The nuclear ribosomal internal transcribed spacer (ITS) region is the most commonly chosen
genetic marker for the molecular identification of fungi in environmental sequencing and …

Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of
numerous samples for high-throughput sequencing (HTS). This approach is routinely used …

The ocean constitutes one of the vastest and richest biomes on our planet. Most recent
estimations, all based on indirect approaches, suggest that there are millions of marine …