During the past three decades, mice, zebrafish, fruit flies, and Caenorhabditis elegans have
been the primary model organisms used for the study of various biological phenomena …

The burden of cardiovascular and metabolic diseases worldwide is staggering. The
emergence of systems approaches in biology promises new therapies, faster and cheaper …

Zebrafish (Danio rerio) is an alternative model system for drug screening, developing new
products, and assessing ecotoxic effects of pollutants and biomonitor species in …

Despite the pre-eminence of the mouse in modelling human disease, several aspects of
murine biology limit its routine use in large-scale genetic and therapeutic screening. Many …

Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has
suffered from three problems: the labor of building complex expression constructs using …

As the incidence of hepatobiliary diseases increases, we must improve our understanding of
the molecular, cellular, and physiological factors that contribute to the pathogenesis of liver …

Zebrafish (Danio rerio) has been a prominent model vertebrate in a variety of biological
disciplines. Substantial information gathered from developmental and genetic research …

Tissue damage can resolve completely through healing and regeneration, or can produce
permanent scarring and loss of function. The response to tissue damage varies across …

The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene
transfer in eukaryotic cells. To overcome these limitations, we have produced murine …

We report here development of a novel gene trap method in zebrafish using the Tol2
transposon system. First, we established a highly efficient transgenesis method in which a …