Tetracyclines were discovered in the 1940s and exhibited activity against a wide range of
microorganisms including gram-positive and gram-negative bacteria, chlamydiae …

Multidrug efflux systems display the ability to transport a variety of structurally unrelated
drugs from a cell and consequently are capable of conferring resistance to a diverse range …

An essential feature of bacterial plasmids is their ability to replicate as autonomous genetic
elements in a controlled way within the host. Therefore, they can be used to explore the …

Tetracycline has been a widely used antibiotic because of its low toxicity and broad
spectrum of activity. However, its clinical usefulness has been declining because of the …

Transfer of antibiotic resistance genes by conjugation is thought to play an important role in
the spread of resistance. Yet virtually no information is available about the extent to which …

We isolated a replication-thermosensitive mutant of the broad-host-range replicon pWV01.
The mutant pVE6002 is fully thermosensitive above 35 degrees C in both gram-negative …

A general system is described that facilitates gene replacements such that the recombinant
strains are not labelled with antibiotic resistance genes. The method is based on the …

Plasmids are and will remain important cloning vehicles for biotechnology. They have also
been associated with the spread of a number of diseases and therefore are a subject of …

The early notion of DNA as a passive target for regulatory proteins has given way to the
realization that higher-order DNA structures and DNA-protein complexes are at the basis of …

Many plasmids from gram-positive bacteria replicate via a single-stranded deoxyribonucleic
acid (ssDNA) intermediate, most probably by a rolling-circle mechanism (these plasmids are …