[HTML][HTML] Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models

D Simão, CM Gomes, PM Alves, C Brito - Biotechnology Advances, 2022 - Elsevier
Abstract Advanced three-dimensional (3D) cell models have proven to be capable of
depicting architectural and microenvironmental features of several tissues. By providing data …

[HTML][HTML] Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo

CJ Peddie, MC Domart, X Snetkov, P O'Toole… - Journal of structural …, 2017 - Elsevier
Super-resolution light microscopy, correlative light and electron microscopy, and volume
electron microscopy are revolutionising the way in which biological samples are examined …

Utilization of integrated correlative light and electron microscopy (iCLEM) for imaging sedimentary organic matter

PC Hackley, BJ Valentine, LM Voortman… - Journal of …, 2017 - Wiley Online Library
We report here a new microscopic technique for imaging and identifying sedimentary
organic matter in geologic materials that combines inverted fluorescence microscopy with …

[HTML][HTML] Correlative microscopy for structural microbiology

SC Howes, RI Koning, AJ Koster - Current opinion in microbiology, 2018 - Elsevier
Highlights•Labelling microbes identifies and locates them in situ by correlative microscopy.•
Correlative microscopy efficiently targets high-resolution electron microscopy.•Investigating …

Preservation of fluorescence signal and imaging optimization for integrated light and electron microscopy

P Baatsen, S Gabarre, K Vints, R Wouters… - Frontiers in Cell and …, 2021 - frontiersin.org
Life science research often needs to define where molecules are located within the complex
environment of a cell or tissue. Genetically encoded fluorescent proteins and or …

Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers

MT Haring, N Liv, AC Zonnevylle, AC Narvaez… - Scientific reports, 2017 - nature.com
In the biological sciences, data from fluorescence and electron microscopy is correlated to
allow fluorescence biomolecule identification within the cellular ultrastructure and/or …

Correlative organelle microscopy: fluorescence guided volume electron microscopy of intracellular processes

SV Loginov, J Fermie, J Fokkema… - Frontiers in Cell and …, 2022 - frontiersin.org
Intracellular processes depend on a strict spatial and temporal organization of proteins and
organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is …

Multimodal Characterization of Resin Embedded and Sliced Polymer Nanoparticles by Means of Tip‐Enhanced Raman Spectroscopy and Force–Distance Curve …

C Höppener, FH Schacher, V Deckert - Small, 2020 - Wiley Online Library
Understanding the property‐function relation of nanoparticles in various application fields
involves determining their physicochemical properties, which is still a remaining challenge …

An easy path for correlative electron and super-resolution light microscopy

D Pinotsi, S Rodighiero, S Campioni, G Csucs - Scientific reports, 2019 - nature.com
A number of new Correlative Light and Electron Microscopy approaches have been
developed over the past years, offering the opportunity to combine the specificity and bio …

The Rényi divergence enables accurate and precise cluster analysis for localization microscopy

AD Staszowska, P Fox-Roberts, LM Hirvonen… - …, 2018 - academic.oup.com
Motivation Clustering analysis is a key technique for quantitatively characterizing structures
in localization microscopy images. To build up accurate information about biological …