Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …

It has been over 20 years since the pioneering work of Arthur Ashkin, and in the intervening
years, the field of optical tweezers has grown tremendously. Optical tweezers are now being …

We review the combinations of optical micro‐manipulation with other techniques and their
classical and emerging applications to non‐contact optical separation and sorting of micro …

Single molecule force spectroscopy is a powerful method that uses the mechanical
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …

Single-molecule micromanipulation techniques traditionally have been developed for
biophysical applications, but they are being increasingly employed in materials science …

The central catalyst in eukaryotic ATP-dependent homologous recombination consists of
RAD51 proteins, polymerized around single-stranded DNA. This nucleoprotein filament …

Rad51 protein (Rad51) is central to recombinational repair of double-strand DNA breaks. It
polymerizes onto DNA and promotes strand exchange between homologous chromosomes …

An essential mechanism for repairing DNA double‐strand breaks is homologous
recombination (HR). One of its core catalysts is human RAD 51 (hRAD 51), which …

Over the past two decades, single-molecule techniques have evolved into robust tools to
study many fundamental biological processes. The combination of optical tweezers with …

摘要光镊采用聚焦的激光束束缚微米, 纳米级的粒子, 具有亚皮牛级的力分辨率和亚毫秒级的
时间响应, 在单分子生物物理中具有广泛的应用. 通过化学耦链将生物大分子连接到高分子微球 …