Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

HH Kang, AH Naing, CK Kim - Biology, 2020 - mdpi.com
Despite the increasing use of protoplasts in plant biotechnology research, shoot
regeneration from protoplasts remains challenging. In this study, we investigated the factors …

Acute lipopolysaccharide (LPS)-induced cell membrane hyperpolarization is independent of voltage gated and calcium activated potassium channels

S McCubbin, A Meade, DA Harrison… - … and Physiology Part C …, 2024 - Elsevier
The gram-negative toxin lipopolysaccharides (LPS) are known to trigger inflammatory
cytokines in mammals, which can result in pathological responses. Upon treatment of …

Hyperpolarization Induced by Lipopolysaccharides but Not by Chloroform Is Inhibited by Doxapram, an Inhibitor of Two-P-Domain K+ Channel (K2P)

RL Cooper, RM Krall - International Journal of Molecular Sciences, 2022 - mdpi.com
Bacterial septicemia is commonly induced by Gram-negative bacteria. The immune
response is triggered in part by the secretion of bacterial endotoxin lipopolysaccharide …

The effects of doxapram blocking the response of gram-negative bacterial toxin (LPS) at glutamatergic synapses

KE Brock, RL Cooper - Biology, 2023 - mdpi.com
Simple Summary The actions of bacterial toxin lipopolysaccharides (LPS) can lead to the
development of illness in humans and animals by triggering an immune system response …

Bacterial lipopolysaccharide hyperpolarizes the membrane potential and is antagonized by the K2p channel blocker doxapram

RM Vacassenno, CN Haddad, RL Cooper - Comparative Biochemistry and …, 2023 - Elsevier
Exposure of Drosophila skeletal muscle to bacterial lipopolysaccharides (LPS) rapidly and
transiently hyperpolarizes membrane potential. However, the mechanism responsible for …

Rapid and Direct Action of Lipopolysaccharide (LPS) on Skeletal Muscle of Larval Drosophila

R Potter, A Meade, S Potter, RL Cooper - Biology, 2021 - mdpi.com
Simple Summary The direct action of a toxin, lipopolysaccharide, which is released from
bacteria, on tissues is still not well understood. Skeletal muscle exposed to …

Bacterial endotoxin lipopolysaccharide enhances synaptic transmission at low-output glutamatergic synapses

A Greenhalgh, O Istas, RL Cooper - Neuroscience Research, 2021 - Elsevier
The endotoxin lipopolysaccharides (LPS), secreted from gram-negative bacteria, has direct
effects on synaptic transmission independent of systemic secondary cytokine responses …

The effects of Gram-positive and Gram-negative bacterial toxins (LTA & LPS) on cardiac function in Drosophila melanogaster larvae

KE Brock, ER Elliott, MO Abul-Khoudoud… - Journal of Insect …, 2023 - Elsevier
The effects of Gram negative and positive bacterial sepsis depend on the type of toxins
released, such as lipopolysaccharides (LPS) or lipoteichoic acid (LTA). Previous studies …

Mutations of γCOP Gene Disturb Drosophila melanogaster Innate Immune Response to Pseudomonas aeruginosa

MC Chifiriuc, AM Bologa, AC Ratiu, A Ionascu… - International Journal of …, 2022 - mdpi.com
Drosophila melanogaster (the fruit fly) is a valuable experimental platform for modeling host–
pathogen interactions. It is also commonly used to define innate immunity pathways and to …

Fluoxetine antagonizes the acute response of LPS: Blocks K2P channels

ER Elliott, RL Cooper - Comparative Biochemistry and Physiology Part C …, 2025 - Elsevier
The channels responsible for maintaining resting membrane potential are known as K2P
(two-P-domain K+ subunit) channels, a subset of which are known to be blocked by …