Ligands for mixed-mode protein chromatography: principles, characteristics and design

G Zhao, XY Dong, Y Sun - Journal of biotechnology, 2009 - Elsevier
Mixed-mode chromatography is a chromatographic method that utilizes more than one form
of interactions between the stationary phase and the solutes in a feed stream. Compared …

[图书][B] Handbook of affinity chromatography

T Kline - 1993 - taylorfrancis.com
Outlining the fundamental principles by which all interactions occur, this reference focuses
on harnessing the biochemistry of bioorganic compounds in order to separate them …

Salt-independent adsorption chromatography: new broad-spectrum affinity methods for protein capture

SC Burton, DRK Harding - Journal of biochemical and biophysical methods, 2001 - Elsevier
The role of chromatography in capture is reviewed in terms of the special requirements
imposed by the processing of very crude feedstocks. Adsorption methods which are not …

One step purification of chymosin by mixed mode chromatography

SC Burton, NW Haggarty… - Biotechnology and …, 1997 - Wiley Online Library
Abstract Mixed mode Sepharose and Perloza bead cellulose matrices were prepared using
various chemistries. These matrices contained hydrophobic (aliphatic and/or aromatic) and …

Removal of dye waste by weak cation-exchange nanofiber membrane immobilized with waste egg white proteins

H Pakalapati, PL Show, JH Chang, BL Liu… - International journal of …, 2020 - Elsevier
In this research, a protein nanofiber membrane (P-COOH-CEW) was developed to treat the
dye waste. Initially, polyacrylonitrile nanofiber membrane (PAN) was prepared by …

Characterization of BSA adsorption on mixed mode adsorbent: I. Equilibrium study in a well-agitated contactor

YK Chang, SY Chou, JL Liu, JC Tasi - Biochemical engineering journal, 2007 - Elsevier
STREAMLINE Direct HST (GE Healthcare Biosciences, 2004) is a newly designed mixed
mode adsorbent, which contains small stainless steel particles and is based on a highly …

Chromatographic resins and methods for using same

SC Burton, NW Haggarty, DRK Harding… - US Patent …, 1997 - Google Patents
Disclosed are rationally designed mixed mode resins which are useful in recovering a target
compound from an aqueous solution and methods for use of such resins. The resins …

Penicillin acylase release from Escherichia coli cells by mechanical cell disruption and permeabilization

LP Fonseca, JMS Cabral - Journal of Chemical Technology & …, 2002 - Wiley Online Library
The release of Penicillin acylase from Escherichia coli cells through mechanical cell
disruption using high‐pressure homogenization and sonication was studied. From these cell …

一种新的生物分离方法——混合模式吸附层析

姚善泾, 高栋, 林东强 - 化工学报, 2007 - hgxb.cip.com.cn
混合模式吸附层析是一种新型的生物分离方法, 其功能配基兼有两种或两种以上的作用模式,
主要为静电和疏水相互作用. 在低盐和高盐条件下, 都可以实现对目标物的有效吸附 …

Characterization of novel mixed-mode protein adsorbents fabricated from benzoyl-modified polyethyleneimine-grafted Sepharose

N Liu, Z Wang, XM Liu, L Yu, Y Sun - Journal of Chromatography A, 2014 - Elsevier
We have previously studied poly (ethylenimine)(PEI)-grafted Sepharose FF resins for ion-
exchange chromatography of bovine serum albumin (BSA), and found the presence of a …